Human NT-proBNP ELISA Kit

$245.00 – $350.00

Related Target	nppb
Species	human
Sample Type	Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	4.56 pg/mL
Array Range	31.25 pg/mL - 2000 pg/mL
Assay Time	3.5 h
Recovery	91% - 119%
Average Recovery	106%

ELISA Kit Detail Information

Related Target	nppb
Species	human
Sample Type	Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	4.56 pg/mL
Array Range	31.25 pg/mL - 2000 pg/mL
Assay Time	3.5 h
Recovery	91% - 119%
Average Recovery	106%
Intra Precision	4.1% - 6.0%
Inter Precision	3.7% - 4.1 %
Plate	Detachable 96-well Plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ Blue Ice Transport
Components	1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Detect Antibody Standard Diluent Streptavidin-HRP Standard Microplate Wells
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human NT-proBNP has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for NT-proBNP are pipetted into the wells and NT-proBNP present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of NT-proBNP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Related Targets

nppb

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