Human Proprotein Convertase 9/PCSK9 ELISA Kit
$245.00 – $350.00
Related Target | |
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Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 10 μL |
Sensitivity | 3.39 pg/mL |
Array Range | 62.5 pg/mL – 4000 pg/mL |
Assay Time | 3.5 h |
Recovery | 94% – 110% |
Average Recovery | 103% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 10 μL |
Sensitivity | 3.39 pg/mL |
Array Range | 62.5 pg/mL – 4000 pg/mL |
Assay Time | 3.5 h |
Recovery | 94% – 110% |
Average Recovery | 103% |
Intra Precision | 1.7% – 7.6% |
Inter Precision | 2.4% – 5.8% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-PCSK9 monoclonal antibody Human PCSK9 freeze-dried standard PCSK9 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human PCSK9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, PCSK9 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of PCSK9 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
PCSK9
PCSK9 Target Infomation Overview
- Target Symbol: PCSK9, proprotein convertase subtilisin/kexin type 9
- Gene Groups: Proprotein convertase subtilisin/kexin family
- Alias: NARC-1; FH3
- Previous Names: HCHOLA3
- Alias Names: hypercholesterolemia, autosomal dominant 3
PCSK9, proprotein convertase subtilisin/kexin type 9 Target Infomation by Species
- Human
- Mouse
- Rat
Human PCSK9 Target Information
- Target Symbol: PCSK9, proprotein convertase subtilisin/kexin type 9
- Alias:
- convertase subtilisin/kexin type 9 preproprotein
- FH3
- FHCL3
- HCHOLA3
- LDLCQ1
- NARC-1
- NARC1
- neural apoptosis regulated convertase 1
- neural apoptosis-regulated convertase 1
- PC9
- proprotein convertase 9
- subtilisin/kexin-like protease PC9
- NCBI_Gene: 255738
- UniProtKB: Q8NBP7
Human PCSK9 Predicted Functions
Enables several functions, including lipoprotein particle receptor binding activity; serine-type endopeptidase activity; and sodium channel inhibitor activity. Involved in several processes, including cholesterol homeostasis; low-density lipoprotein particle receptor catabolic process; and regulation of receptor-mediated endocytosis. Located in several cellular components, including Golgi apparatus; endosome; and perinuclear region of cytoplasm. Part of PCSK9-AnxA2 complex and PCSK9-LDLR complex. Implicated in familial hypercholesterolemia and hypobetalipoproteinemia.
Mouse Pcsk9 Target Information
- Target Symbol: Pcsk9, proprotein convertase subtilisin/kexin type 9
- Alias:
- AI415265
- AI747682
- expressed sequence AI415265
- expressed sequence AI747682
- Narc1
- NCBI_Gene: 100102
Mouse Pcsk9 Predicted Functions
Enables apolipoprotein binding activity; lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including cellular response to insulin stimulus; cellular response to starvation; and regulation of neuron apoptotic process. Acts upstream of or within several processes, including low-density lipoprotein receptor particle metabolic process; regulation of low-density lipoprotein particle receptor catabolic process; and triglyceride metabolic process. Located in COPII-coated ER to Golgi transport vesicle; endoplasmic reticulum; and extracellular space. Is expressed in several structures, including alimentary system; cerebellum; genitourinary system; liver; and telencephalon. Human ortholog(s) of this gene implicated in familial hypercholesterolemia and hypobetalipoproteinemia. Orthologous to human PCSK9 (proprotein convertase subtilisin/kexin type 9).
Rat Pcsk9 Target Information
- Target Symbol: Pcsk9, proprotein convertase subtilisin/kexin type 9
- Alias:
- NARC-1
- Narc1
- neural apoptosis regulated convertase 1
- neural apoptosis-regulated convertase 1
- PC9
- proprotein convertase 9
- proprotein convertase PC9
- subtilisin/kexin-like protease PC9
- NCBI_Gene: 298296
- UniProtKB: P59996
Rat Pcsk9 Predicted Functions
Predicted to enable several functions, including lipoprotein particle binding activity; lipoprotein particle receptor binding activity; and serine-type endopeptidase activity. Involved in several processes, including cellular response to insulin stimulus; cholesterol homeostasis; and protein autoprocessing. Located in extracellular space and rough endoplasmic reticulum. Human ortholog(s) of this gene implicated in familial hypercholesterolemia and hypobetalipoproteinemia. Orthologous to human PCSK9 (proprotein convertase subtilisin/kexin type 9).
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