Human sCD163 ELISA Kit

$350.00$450.00

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TARGETSCD163
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.39 pg/mL
Range62.50 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery88% – 110%
Average Recovery0.95
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity4.39 pg/mL
Range62.50 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery88% – 110%
Average Recovery0.95
Intra Precision4.1% – 5.6%
Inter-Precision2.7%-6.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-sCD163A monoclonal antibody
Human sCD163A freeze-dried standard
sCD163A detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human sCD163 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, sCD163 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of sCD163 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CD163

CD163 Target Infomation Overview

  • Target Symbol: CD163, CD163 molecule
  • Gene Groups: CD molecules; Scavenger receptors; Scavenger receptor cysteine rich domain containing
  • Alias: M130; MM130; SCARI1
  • Alias Names: CD163 antigen

CD163, CD163 molecule Target Infomation by Species

HumanMouseRat

Human CD163 Target Information

  • Target Symbol: CD163, CD163 molecule
  • Alias:
    • cluster of differentiation 163
    • hemoglobin scavenger receptor
    • M130
    • macrophage-associated antigen
    • MM130
    • SCARI1
    • scavenger receptor cysteine-rich type 1 protein M130
  • NCBI_Gene: 9332
  • UniProtKB: Q86VB7

Human CD163 Predicted Functions

Enables scaffold protein binding activity. Predicted to be involved in acute-phase response and endocytosis. Located in external side of plasma membrane. Biomarker of several diseases, including Ebola hemorrhagic fever; Whipple disease; fatty liver disease (multiple); leishmaniasis (multiple); and lung disease (multiple).

Mouse Cd163 Target Information

Mouse Cd163 Predicted Functions

Predicted to enable scaffold protein binding activity and scavenger receptor activity. Predicted to act upstream of or within acute-phase response. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Is expressed in several structures, including gut; immune system; male reproductive gland or organ; olfactory lobe; and quadriceps femoris muscle. Orthologous to human CD163 (CD163 molecule).

Rat Cd163 Target Information

Rat Cd163 Predicted Functions

Predicted to enable scaffold protein binding activity. Predicted to be involved in endocytosis. Predicted to be located in plasma membrane. Predicted to be active in external side of plasma membrane. Biomarker of uveitis. Orthologous to human CD163 (CD163 molecule).

CD163 Target News

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