Human Serpin E1/PAI-1 ELISA Kit

$350.00$450.00

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TARGETSSERPINE1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity1.53 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery80% – 118%
Average Recovery0.9
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity1.53 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery80% – 118%
Average Recovery0.9
Intra Precision2.3% – 4.8%
Inter-Precision2.3% – 2.6%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Serpin E1/PAI-1 monoclonal antibody
Human Serpin E1/PAI-1 freeze-dried standard
Serpin E1/PAI-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Serpin E1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Serpin E1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Serpin E1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

SERPINE1

SERPINE1 Target Infomation Overview

  • Target Symbol: SERPINE1, serpin family E member 1
  • Gene Groups: Serpin peptidase inhibitors
  • Alias: PAI
  • Previous Names: PLANH1; PAI1
  • Alias Names: plasminogen activator inhibitor, type I; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

SERPINE1, serpin family E member 1 Target Infomation by Species

HumanMouseRat

Human SERPINE1 Target Information

  • Target Symbol: SERPINE1, serpin family E member 1
  • Alias:
    • endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1
    • PLANH1
    • plasminogen activator inhibitor 1
    • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
    • serpin E1
    • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • NCBI_Gene: 5054
  • UniProtKB: P05121

Human SERPINE1 Predicted Functions

Enables protease binding activity; serine-type endopeptidase inhibitor activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of proteolysis; regulation of angiogenesis; and response to bacterium. Located in collagen-containing extracellular matrix and extracellular exosome. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetic retinopathy (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetic retinopathy (multiple); lung disease (multiple); and sleep apnea (multiple).

Mouse Serpine1 Target Information

Mouse Serpine1 Predicted Functions

Enables serine-type endopeptidase inhibitor activity. Involved in defense response to Gram-negative bacterium; negative regulation of plasminogen activation; and regulation of angiogenesis. Acts upstream of or within several processes, including cellular response to transforming growth factor beta stimulus; placenta development; and regulation of angiogenesis. Located in extracellular space. Is expressed in several structures, including aorta; extraembryonic component; liver; oocyte; and placenta. Used to study Coronavirus infectious disease. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetic retinopathy (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human SERPINE1 (serpin family E member 1).

Rat Serpine1 Target Information

  • Target Symbol: Serpine1, serpin family E member 1
  • Alias:
    • endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • Pai1
    • PAI1A
    • Pai1aa
    • Planh
    • Plasminogen activator inhibitor
    • plasminogen activator inhibitor 1
    • RATPAI1A
    • serine (or cysteine) peptidase inhibitor, clade E, member 1
    • serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
    • serine (or cysteine) proteinase inhibitor, clade E, member 1
    • serine (or cysteine) proteinase inhibitor, member 1
    • serpin E1
    • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • NCBI_Gene: 24617

Rat Serpine1 Predicted Functions

Enables protease binding activity and serine-type endopeptidase inhibitor activity. Involved in several processes, including cellular response to growth factor stimulus; cellular response to hormone stimulus; and cellular response to organonitrogen compound. Located in chromaffin granule and extracellular space. Used to study several diseases, including artery disease (multiple); cerebrovascular disease (multiple); diabetes mellitus (multiple); end stage renal disease; and renal fibrosis. Biomarker of several diseases, including acute necrotizing pancreatitis; glomerulosclerosis (multiple); lung disease (multiple); middle cerebral artery infarction; and muscular atrophy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetic retinopathy (multiple); liver disease (multiple); and lung disease (multiple). Orthologous to human SERPINE1 (serpin family E member 1).

SERPINE1 Target News

    5.8
    Helicobacter pylori-induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis
    Cancer Cell International
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Obesity Diabetes
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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