Human SP-D ELISA?Kit

$245.00 – $350.00

Related Target	SFTPD
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.45 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	94% – 120%
Average Recovery	105%

ELISA Kit Detail Information

Related Target	SFTPD
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.45 pg/mL
Array Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	94% – 120%
Average Recovery	105%
Intra Precision	5.2% – 6.1%
Inter Precision	3.2% – 8.0%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-SP-D monoclonal antibody Human SP-D freeze-dried standard SP-D detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human SP-D antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, SP-D present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of SP-D in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

SFTPD

SFTPD Target Infomation Overview

Target Symbol: SFTPD, surfactant protein D
Gene Groups: Collectins; C-type lectin domain containing
Alias: SP-D; COLEC7
Previous Names: SFTP4
Alias Names: surfactant, pulmonary-associated protein D

SFTPD, surfactant protein D Target Infomation by Species

Human
Mouse
Rat

Human SFTPD Target Information

Target Symbol: SFTPD, surfactant protein D
Alias:
- COLEC7
- collectin 7
- collectin-7
- lung surfactant protein D
- PSP-D
- pulmonary surfactant apoprotein
- pulmonary surfactant-associated protein D
- SFTP4
- SP-D
- surfactant-associated protein, pulmonary 4
- surfactant, pulmonary-associated protein D
NCBI_Gene: 6441
UniProtKB: P35247

Human SFTPD Predicted Functions

Predicted to enable identical protein binding activity. Involved in several processes, including induction of bacterial agglutination; negative regulation by host of symbiont catalytic activity; and surfactant homeostasis. Predicted to be located in cytoplasm. Predicted to be part of collagen trimer. Predicted to be active in extracellular space; multivesicular body; and rough endoplasmic reticulum. Implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Biomarker of several diseases, including cystic fibrosis; lung disease (multiple); respiratory syncytial virus infectious disease; sarcoidosis; and systemic lupus erythematosus.

Mouse Sftpd Target Information

Target Symbol: Sftpd, surfactant associated protein D
Alias:
- AI573415
- expressed sequence AI573415
- Sfpd
- Sftp4
- SP-D
- surfactant-associated protein 4
NCBI_Gene: 20390
- UniprotKB: P50404

Mouse Sftpd Predicted Functions

Enables identical protein binding activity. Predicted to be involved in several processes, including induction of bacterial agglutination; negative regulation of interleukin-2 production; and regulation of phagocytosis. Predicted to act upstream of or within innate immune response and respiratory gaseous exchange by respiratory system. Located in cytoplasm. Is expressed in lung; otic capsule; and skeleton. Human ortholog(s) of this gene implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Orthologous to human SFTPD (surfactant protein D).

Rat Sftpd Target Information

Target Symbol: Sftpd, surfactant protein D
Alias:
- CP4
- lung surfactant protein D
- PSP-D
- pulmonary surfactant protein D
- pulmonary surfactant-associated protein D
- SP-D
- SPD
- surfactant associated protein D
- surfactant pulmonary-associated protein D
- surfactant, pulmonary-associated protein D
NCBI_Gene: 25350

Rat Sftpd Predicted Functions

Enables identical protein binding activity; lipopolysaccharide binding activity; and monosaccharide binding activity. Involved in several processes, including negative regulation of interleukin-2 production; opsonization; and regulation of phagocytosis. Located in extracellular space; multivesicular body; and rough endoplasmic reticulum. Biomarker of Coronavirus infectious disease; asthma; bacterial pneumonia; lung disease; and newborn respiratory distress syndrome. Human ortholog(s) of this gene implicated in asthma; lung disease (multiple); respiratory syncytial virus infectious disease; and rhinitis. Orthologous to human SFTPD (surfactant protein D).

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