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Human TIMP-4 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 2.07 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 95% – 114% |
| Average Recovery | 1.02 |
| Intra Precision | 4.0% – 4.8% |
| Inter-Precision | 4.6% – 4.8% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-4 monoclonal antibody Human TIMP-4 freeze-dried standard TIMP-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TIMP4
TIMP4 Target Infomation Overview
- Target Symbol: TIMP4, TIMP metallopeptidase inhibitor 4
- Gene Groups: Tissue inhibitor of metallopeptidases
- Alias Names: tissue inhibitor of metalloproteinase 4
TIMP4, TIMP metallopeptidase inhibitor 4 Target Infomation by Species
HumanMouseRat
Human TIMP4 Target Information
- Target Symbol: TIMP4, TIMP metallopeptidase inhibitor 4
- Alias:
- metalloproteinase inhibitor 4
- TIMP-4
- tissue inhibitor of metalloproteinase 4
- tissue inhibitor of metalloproteinases 4
- NCBI_Gene: 7079
- UniProtKB: Q99727
Human TIMP4 Predicted Functions
Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Predicted to be involved in negative regulation of proteolysis; response to cytokine; and response to hormone. Predicted to act upstream of or within Notch signaling pathway. Predicted to be located in sarcomere. Predicted to be active in extracellular matrix and extracellular space. Implicated in nephroblastoma. Biomarker of ductal carcinoma in situ; female reproductive organ cancer (multiple); prostate carcinoma in situ; and renal cell carcinoma.
Mouse Timp4 Target Information
- Target Symbol: Timp4, tissue inhibitor of metalloproteinase 4
- Alias:
- TIMP-4
- NCBI_Gene: 110595
Mouse Timp4 Predicted Functions
Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Acts upstream of or within Notch signaling pathway. Predicted to be located in sarcomere. Predicted to be active in extracellular matrix and extracellular space. Is expressed in several structures, including brain ventricular layer; heart; ovary; skin; and ventral grey horn. Human ortholog(s) of this gene implicated in nephroblastoma. Orthologous to human TIMP4 (TIMP metallopeptidase inhibitor 4).
Rat Timp4 Target Information
- Target Symbol: Timp4, TIMP metallopeptidase inhibitor 4
- Alias:
- LOC680130
- metalloproteinase inhibitor 4
- similar to Metalloproteinase inhibitor 4 precursor (TIMP-4) (Tissue inhibitor of metalloproteinases-4)
- TIMP-4
- Timp4_mapped
- tissue inhibitor of metalloproteinase 4
- tissue inhibitor of metalloproteinase 4 (mapped)
- tissue inhibitor of metalloproteinases 4
- NCBI_Gene: 680130
- UniProtKB: P81556
Rat Timp4 Predicted Functions
Predicted to enable metalloendopeptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in several processes, including ovulation cycle; response to lipopolysaccharide; and response to peptide hormone. Located in extracellular space and sarcomere. Biomarker of abdominal aortic aneurysm; congestive heart failure; and renal hypertension. Human ortholog(s) of this gene implicated in nephroblastoma. Orthologous to human TIMP4 (TIMP metallopeptidase inhibitor 4).
