Human TIMP-4 ELISA Kit

$350.00$450.00

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TARGETSTIMP4
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.07 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery95% – 114%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume10 μL
Sensitivity2.07 pg/mL
Range62.5 pg/mL – 4000 pg/mL
Assay Time3.5 h
Recovery95% – 114%
Average Recovery1.02
Intra Precision4.0% – 4.8%
Inter-Precision4.6% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-4 monoclonal antibody
Human TIMP-4 freeze-dried standard
TIMP-4 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TIMP4

TIMP4 Target Infomation Overview

  • Target Symbol: TIMP4, TIMP metallopeptidase inhibitor 4
  • Gene Groups: Tissue inhibitor of metallopeptidases
  • Alias Names: tissue inhibitor of metalloproteinase 4

TIMP4, TIMP metallopeptidase inhibitor 4 Target Infomation by Species

HumanMouseRat

Human TIMP4 Target Information

  • Target Symbol: TIMP4, TIMP metallopeptidase inhibitor 4
  • Alias:
    • metalloproteinase inhibitor 4
    • TIMP-4
    • tissue inhibitor of metalloproteinase 4
    • tissue inhibitor of metalloproteinases 4
  • NCBI_Gene: 7079
  • UniProtKB: Q99727

Human TIMP4 Predicted Functions

Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Predicted to be involved in negative regulation of proteolysis; response to cytokine; and response to hormone. Predicted to act upstream of or within Notch signaling pathway. Predicted to be located in sarcomere. Predicted to be active in extracellular matrix and extracellular space. Implicated in nephroblastoma. Biomarker of ductal carcinoma in situ; female reproductive organ cancer (multiple); prostate carcinoma in situ; and renal cell carcinoma.

Mouse Timp4 Target Information

Mouse Timp4 Predicted Functions

Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Acts upstream of or within Notch signaling pathway. Predicted to be located in sarcomere. Predicted to be active in extracellular matrix and extracellular space. Is expressed in several structures, including brain ventricular layer; heart; ovary; skin; and ventral grey horn. Human ortholog(s) of this gene implicated in nephroblastoma. Orthologous to human TIMP4 (TIMP metallopeptidase inhibitor 4).

Rat Timp4 Target Information

  • Target Symbol: Timp4, TIMP metallopeptidase inhibitor 4
  • Alias:
    • LOC680130
    • metalloproteinase inhibitor 4
    • similar to Metalloproteinase inhibitor 4 precursor (TIMP-4) (Tissue inhibitor of metalloproteinases-4)
    • TIMP-4
    • Timp4_mapped
    • tissue inhibitor of metalloproteinase 4
    • tissue inhibitor of metalloproteinase 4 (mapped)
    • tissue inhibitor of metalloproteinases 4
  • NCBI_Gene: 680130
  • UniProtKB: P81556

Rat Timp4 Predicted Functions

Predicted to enable metalloendopeptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in several processes, including ovulation cycle; response to lipopolysaccharide; and response to peptide hormone. Located in extracellular space and sarcomere. Biomarker of abdominal aortic aneurysm; congestive heart failure; and renal hypertension. Human ortholog(s) of this gene implicated in nephroblastoma. Orthologous to human TIMP4 (TIMP metallopeptidase inhibitor 4).

TIMP4 Target News

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