Human TIMP-4 ELISA Kit

$245.00 – $350.00

Related Target	TIMP4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.07 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	95% – 114%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	TIMP4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	10 μL
Sensitivity	2.07 pg/mL
Array Range	62.5 pg/mL – 4000 pg/mL
Assay Time	3.5 h
Recovery	95% – 114%
Average Recovery	102%
Intra Precision	4.0% – 4.8%
Inter Precision	4.6% – 4.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TIMP-4 monoclonal antibody Human TIMP-4 freeze-dried standard TIMP-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human TIMP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TIMP-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TIMP-4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TIMP4

TIMP4 Target Infomation Overview

Target Symbol: TIMP4, TIMP metallopeptidase inhibitor 4
Gene Groups: Tissue inhibitor of metallopeptidases
Alias Names: tissue inhibitor of metalloproteinase 4

TIMP4, TIMP metallopeptidase inhibitor 4 Target Infomation by Species

Human
Mouse
Rat

Human TIMP4 Target Information

Target Symbol: TIMP4, TIMP metallopeptidase inhibitor 4
Alias:
- metalloproteinase inhibitor 4
- TIMP-4
- tissue inhibitor of metalloproteinase 4
- tissue inhibitor of metalloproteinases 4
NCBI_Gene: 7079
UniProtKB: Q99727

Human TIMP4 Predicted Functions

Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Predicted to be involved in negative regulation of proteolysis; response to cytokine; and response to hormone. Predicted to act upstream of or within Notch signaling pathway. Predicted to be located in sarcomere. Predicted to be active in extracellular matrix and extracellular space. Implicated in nephroblastoma. Biomarker of ductal carcinoma in situ; female reproductive organ cancer (multiple); prostate carcinoma in situ; and renal cell carcinoma.

Mouse Timp4 Target Information

Target Symbol: Timp4, tissue inhibitor of metalloproteinase 4
Alias:
- TIMP-4
NCBI_Gene: 110595

Mouse Timp4 Predicted Functions

Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Acts upstream of or within Notch signaling pathway. Predicted to be located in sarcomere. Predicted to be active in extracellular matrix and extracellular space. Is expressed in several structures, including brain ventricular layer; heart; ovary; skin; and ventral grey horn. Human ortholog(s) of this gene implicated in nephroblastoma. Orthologous to human TIMP4 (TIMP metallopeptidase inhibitor 4).

Rat Timp4 Target Information

Target Symbol: Timp4, TIMP metallopeptidase inhibitor 4
Alias:
- LOC680130
- metalloproteinase inhibitor 4
- similar to Metalloproteinase inhibitor 4 precursor (TIMP-4) (Tissue inhibitor of metalloproteinases-4)
- TIMP-4
- Timp4_mapped
- tissue inhibitor of metalloproteinase 4
- tissue inhibitor of metalloproteinase 4 (mapped)
- tissue inhibitor of metalloproteinases 4
NCBI_Gene: 680130
UniProtKB: P81556

Rat Timp4 Predicted Functions

Predicted to enable metalloendopeptidase inhibitor activity; protease binding activity; and zinc ion binding activity. Involved in several processes, including ovulation cycle; response to lipopolysaccharide; and response to peptide hormone. Located in extracellular space and sarcomere. Biomarker of abdominal aortic aneurysm; congestive heart failure; and renal hypertension. Human ortholog(s) of this gene implicated in nephroblastoma. Orthologous to human TIMP4 (TIMP metallopeptidase inhibitor 4).

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