EK2311

Mouse CD30/TNFRSF8 ELISA Kit

$245.00$350.00

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity0.88 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery92% – 118%
Average Recovery106%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity0.88 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery92% – 118%
Average Recovery106%
Intra Precision3.5% – 4.7%
Inter Precision1.1% - 2.8%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CD30 monoclonal antibody
Human CD30 freeze-dried standard
Angiotensinogen/AGT/ SerpinA8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BMP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, CD30 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CD30 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

TNFRSF8

TNFRSF8 Target Infomation Overview

  • Target Symbol: TNFRSF8, TNF receptor superfamily member 8
  • Gene Groups: CD molecules; Tumor necrosis factor receptor superfamily
  • Alias: KI-1
  • Previous Names: CD30; D1S166E
  • Alias Names: tumor necrosis factor receptor superfamily, member 8

TNFRSF8, TNF receptor superfamily member 8 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human TNFRSF8 Target Information

  • Target Symbol: TNFRSF8, TNF receptor superfamily member 8
  • Alias:
    • CD30
    • CD30 antigen
    • CD30L receptor
    • cytokine receptor CD30
    • D1S166E
    • Ki-1
    • Ki-1 antigen
    • lymphocyte activation antigen CD30
    • tumor necrosis factor receptor superfamily member 8
    • tumor necrosis factor receptor superfamily, member 8
  • NCBI_Gene: 943
  • UniProtKB: P28908

Human TNFRSF8 Predicted Functions

Predicted to enable transmembrane signaling receptor activity. Involved in cellular response to mechanical stimulus; positive regulation of apoptotic process; and positive regulation of tumor necrosis factor superfamily cytokine production. Located in plasma membrane.

Mouse Tnfrsf8 Target Information

Mouse Tnfrsf8 Predicted Functions

Predicted to enable transmembrane signaling receptor activity. Predicted to be involved in positive regulation of NF-kappaB transcription factor activity; positive regulation of apoptotic process; and positive regulation of tumor necrosis factor superfamily cytokine production. Predicted to be located in plasma membrane. Is expressed in several structures, including adrenal gland; brain; female reproductive system; intestine; and thymus. Orthologous to human TNFRSF8 (TNF receptor superfamily member 8).

Rat Tnfrsf8 Target Information

  • Target Symbol: Tnfrsf8, TNF receptor superfamily member 8
  • Alias:
    • Cd30
    • CD30L receptor
    • lymphocyte activation antigen CD30
    • tumor necrosis factor receptor superfamily member 8
    • tumor necrosis factor receptor superfamily, member 8
    • Tumor necrosis factor superfamily member 8
    • tumor necrosis factor superfamily member CD30
    • tumor necrosis factor superfamily, member CD30
  • NCBI_Gene: 25069

Rat Tnfrsf8 Predicted Functions

Predicted to enable transmembrane signaling receptor activity. Involved in positive regulation of NF-kappaB transcription factor activity. Predicted to be located in plasma membrane. Orthologous to human TNFRSF8 (TNF receptor superfamily member 8).

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