Mouse Dkk-1 ELISA Kit

$350.00$450.00

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TARGETSDKK1
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity3.14 pg/mL
Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery80% – 118%
Average Recovery1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity3.14 pg/mL
Range93.75 pg/mL – 6000 pg/mL
Assay Time3.5 h
Recovery80% – 118%
Average Recovery1
Intra Precision2.7% – 4.2%
Inter-Precision2.5% – 4.2%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Dkk-1 monoclonal antibody
Mouse Dkk-1 freeze-dried standard
Dkk-1 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Dkk-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Dkk-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Dkk-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

DKK1

DKK1 Target Infomation Overview

  • Target Symbol: DKK1, dickkopf WNT signaling pathway inhibitor 1
  • Alias: SK; DKK-1
  • Alias Names: dickkopf (Xenopus laevis) homolog 1; dickkopf 1 homolog (Xenopus laevis)

DKK1, dickkopf WNT signaling pathway inhibitor 1 Target Infomation by Species

HumanMouseRat

Human DKK1 Target Information

  • Target Symbol: DKK1, dickkopf WNT signaling pathway inhibitor 1
  • Alias:
    • dickkopf 1 homolog
    • dickkopf related protein-1
    • dickkopf-1 like
    • dickkopf-like protein 1
    • dickkopf-related protein 1
    • DKK-1
    • hDkk-1
    • SK
  • NCBI_Gene: 22943
  • UniProtKB: O94907

Human DKK1 Predicted Functions

Enables co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in several processes, including regulation of cell fate specification; regulation of gene expression; and regulation of protein phosphorylation. Located in extracellular space and plasma membrane. Implicated in anodontia.

Mouse Dkk1 Target Information

Mouse Dkk1 Predicted Functions

Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in several processes, including modulation of age-related behavioral decline; negative regulation of cellular component organization; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including Wnt signaling pathway involved in somitogenesis; embryonic morphogenesis; and negative regulation of signal transduction. Predicted to be located in extracellular region and plasma membrane. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; eye; genitourinary system; and nervous system. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).

Rat Dkk1 Target Information

  • Target Symbol: Dkk1, dickkopf WNT signaling pathway inhibitor 1
  • Alias:
    • dickkopf 1 homolog
    • dickkopf 1 homolog (Xenopus laevis)
    • dickkopf homolog 1
    • dickkopf homolog 1 (Xenopus laevis)
    • dickkopf-like protein 1
    • dickkopf-related protein 1
    • LOC293897
    • NEWGENE_1307313
  • NCBI_Gene: 293897
  • UniProtKB: D3Z9J1

Rat Dkk1 Predicted Functions

Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in negative regulation of ossification and positive regulation of neuron death. Located in extracellular space. Used to study visual epilepsy. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).

DKK1 Target News

    Osteoarthrosis & Rheumatoid Arthritis
    Osteoporosis

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