Mouse Galectin-1 ELISA Kit

$245.00 – $350.00

Related Target	LGALS1
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.11 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	71% – 117%
Average Recovery	98%

ELISA Kit Detail Information

Related Target	LGALS1
Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	1.11 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	71% – 117%
Average Recovery	98%
Intra Precision	2.4% – 3.9%
Inter Precision	2.7% - 4.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Galectin-1 monoclonal antibody Mouse Galectin-1 freeze-dried standard Mouse Galectin-1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse Galectin-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Galectin-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Galectin-1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

LGALS1

LGALS1 Target Infomation Overview

Target Symbol: LGALS1, galectin 1
Gene Groups: Receptor ligands; Galectins
Alias: GBP
Alias Names: lectin, galactoside-binding, soluble, 1

LGALS1, galectin 1 Target Infomation by Species

Human
Mouse
Rat

Human LGALS1 Target Information

Target Symbol: LGALS1, galectin 1
Alias:
- 14 kDa laminin-binding protein
- 14 kDa lectin
- beta-galactoside-binding lectin L-14-I
- beta-galactoside-binding protein 14kDa
- DKFZp686E23103
- gal-1
- GAL1
- galaptin
- galectin-1
- GBP
- HBL
- HLBP14
- HPL
- lactose-binding lectin 1
- lectin, galactoside binding soluble 1
- lectin, galactoside-binding, soluble, 1
- putative MAPK-activating protein PM12
- S-Lac lectin 1
NCBI_Gene: 3956
UniProtKB: P09382

Human LGALS1 Predicted Functions

Enables RNA binding activity. Involved in positive regulation of viral entry into host cell. Located in extracellular exosome. Colocalizes with collagen-containing extracellular matrix.

Mouse Lgals1 Target Information

Target Symbol: Lgals1, lectin, galactose binding, soluble 1
Alias:
- AA410090
- beta galactoside binding protein
- expressed sequence AA410090
- Gal-1
- Galbp
- galectin-1
- L14
- lactose binding soluble lectin, 14 kDa
- Lect14
NCBI_Gene: 16852
- UniprotKB: P16045
- UniprotKB: A0A2R8VHJ0

Mouse Lgals1 Predicted Functions

Enables carbohydrate binding activity. Acts upstream of or within T cell costimulation; myoblast differentiation; and plasma cell differentiation. Located in extracellular space. Is expressed in several structures, including embryo mesenchyme; genitourinary system; integumental system; main olfactory bulb; and olfactory system. Orthologous to human LGALS1 (galectin 1).

Rat Lgals1 Target Information

Target Symbol: Lgals1, galectin 1
Alias:
- 14 kDa lectin
- beta-galactoside-binding lectin
- beta-galactoside-binding lectin L-14-I
- gal-1
- galaptin
- galectin-1
- lactose-binding lectin 1
- lectin, galactose binding, soluble 1
- lectin, galactoside-binding, soluble, 1
- RL 14.5
- S-Lac lectin 1
NCBI_Gene: 56646
UniProtKB: P11762

Rat Lgals1 Predicted Functions

Enables identical protein binding activity; lactose binding activity; and laminin binding activity. Involved in several processes, including cellular response to glucose stimulus; positive regulation of erythrocyte aggregation; and response to isolation stress. Located in cell surface; cytosol; and nucleus. Biomarker of B-cell lymphoma; extrahepatic cholestasis; and lipoid nephrosis. Orthologous to human LGALS1 (galectin 1).

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