Mouse Ig Isotyping ELISA Kit

$299.00 – $419.00

Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity
Array Range
Assay Time	3.5 h
Recovery
Average Recovery

SKU: EK279 Category: ELISA Kit

ELISA Kit Detail Information

Species	mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity
Array Range
Assay Time	3.5 h
Recovery
Average Recovery
Intra Precision
Inter Precision
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	Pre-coated ELISA plate detect Antibody Positive control 10×Assay Buffer Substrate TMB Stop Solution 20×Washing Buffer Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique. Specific monoclonal antibodies against mouse immunoglobulin subtypes are precoated on a high-affinity ELISA plate. The ELISA plate wells are added with test samples, positive controls, and HRP-conjugated detection antibodies. After incubation, the immunoglobulins present in the samples bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, TMB chromogenic substrate is added for color development while avoiding light. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol

Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.

ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects

Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.