EK279

Mouse Ig Isotyping ELISA Kit

$245.00$350.00

Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity
Array Range
Assay Time3.5 h
Recovery
Average Recovery

ELISA Kit Detail Information

Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL (diluent)
Sensitivity
Array Range
Assay Time3.5 h
Recovery
Average Recovery
Intra Precision
Inter Precision
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
ComponentsPre-coated ELISA plate
detect Antibody
Positive control
10×Assay Buffer
Substrate TMB
Stop Solution
20×Washing Buffer
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique. Specific monoclonal antibodies against mouse immunoglobulin subtypes are precoated on a high-affinity ELISA plate. The ELISA plate wells are added with test samples, positive controls, and HRP-conjugated detection antibodies. After incubation, the immunoglobulins present in the samples bind to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, TMB chromogenic substrate is added for color development while avoiding light. The reaction is terminated by adding a stop solution, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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