Mouse IL-12p70 High Sensitivity ELISA Kit

High Sensitivity ELISA

$380.00 – $480.00

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Datasheet

SDS File

TARGETS	IL12B
SPECIES	Mouse
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.57 pg/mL
Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	92% – 115%
Average Recovery	1.04

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.57 pg/mL
Range	7.81 pg/mL – 500 pg/mL
Assay Time	3.5 h
Recovery	92% – 115%
Average Recovery	1.04
Intra Precision	2.2% – 3.1%
Inter-Precision	2.6% – 3.1%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-12p70 monoclonal antibody Mouse IL-12p70 freeze-dried standard IL-12p70 detect Antibody Standard Diluent HRP-labeled streptavidin Signal enhancer concentrate Signal enhancer diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-12p70 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the IL-12p70 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-12p70 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

IL12B

IL12B Target Infomation Overview

Target Symbol: IL12B, interleukin 12B
Gene Groups: Immunoglobulin like domain containing; Interleukins
Alias: CLMF; IL-12B; NKSF; CLMF2
Previous Names: NKSF2
Alias Names: natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B, interleukin 12B Target Infomation by Species

HumanMouseRat

Human IL12B Target Information

Target Symbol: IL12B, interleukin 12B
Alias:
- CLMF
- CLMF p40
- CLMF2
- cytotoxic lymphocyte maturation factor 40 kDa subunit
- IL-12 subunit p40
- IL-12B
- IL12, subunit p40
- IMD28
- IMD29
- interleukin 12, p40
- interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2
- interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
- interleukin-12 beta chain
- interleukin-12 subunit beta
- natural killer cell stimulatory factor, 40 kD subunit
- NK cell stimulatory factor chain 2
- NKSF
- NKSF2
NCBI_Gene: 3593
UniProtKB: P29460

Human IL12B Predicted Functions

Enables identical protein binding activity; interleukin-12 alpha subunit binding activity; and protein heterodimerization activity. Contributes to cytokine activity; growth factor activity; and interleukin-23 receptor binding activity. Involved in several processes, including positive regulation of lymphocyte proliferation; positive regulation of peptidyl-tyrosine phosphorylation; and regulation of cytokine production. Acts upstream of or within negative regulation of protein secretion. Part of interleukin-12 complex and interleukin-23 complex. Implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Biomarker of anogenital venereal wart.

Mouse Il12b Target Information

Target Symbol: Il12b, interleukin 12b
Alias:
- IL-12 p40
- Il-12b
- Il-12p40
- IL-23 subunit p40
NCBI_Gene: 16160
- UniprotKB: P43432
- UniprotKB: Q3ZAX5

Mouse Il12b Predicted Functions

Enables identical protein binding activity and interleukin-12 alpha subunit binding activity. Contributes to cytokine activity and cytokine receptor binding activity. Involved in positive regulation of T cell mediated cytotoxicity; positive regulation of T-helper 1 type immune response; and positive regulation of defense response to virus by host. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; defense response to other organism; and positive regulation of interferon-gamma production. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex and interleukin-23 complex. Is expressed in colon. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).

Rat Il12b Target Information

Target Symbol: Il12b, interleukin 12B
Alias:
- Il12
- Interleukin 12
- interleukin-12 p40
- interleukin-12 p40 precursor
- interleukin-12 subunit beta
NCBI_Gene: 64546

Rat Il12b Predicted Functions

Enables interleukin-12 alpha subunit binding activity. Involved in natural killer cell activation involved in immune response and sensory perception of pain. Located in extracellular space. Part of interleukin-12 complex. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).