Powered by BiozMouse IL-15/IL-15R Complex ELISA Kit
$299.00 – $419.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | Serum, plasma: 20 μL, cell culture supernatant: 100 μL |
| Sensitivity | 0.65 pg/mL |
| Array Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 88% – 112% |
| Average Recovery | 101% |
| Intra Precision | 2.4% – 4.5% |
| Inter Precision | 3.0% – 6.2% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-15/IL-15R monoclonal antibody Mouse IL-15/IL-15R freeze-dried standard IL-15/IL-15R detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IL-15/IL-15R antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-15/IL-15R present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-15/IL-15R in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IL15
IL15 Target Infomation Overview
- Target Symbol: IL15, interleukin 15
- Gene Groups: Interleukins
- Alias: IL-15; MGC9721
IL15, interleukin 15 Target Infomation by Species
- Human
- Mouse
- Rat
Human IL15 Target Information
- Target Symbol: IL15, interleukin 15
- Alias:
- IL-15
- interleukin-15
- MGC9721
- NCBI_Gene: 3600
- UniProtKB: P40933
Human IL15 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including cell surface receptor signaling pathway; macrophage differentiation; and positive regulation of macromolecule metabolic process. Located in cytosol and nuclear speck. Implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Biomarker of several diseases, including artery disease (multiple); brain infarction; lung disease (multiple); obesity; and viral infectious disease (multiple).
Mouse Il15 Target Information
- Target Symbol: Il15, interleukin 15
- Alias:
- AI503618
- expressed sequence AI503618
- NCBI_Gene: 16168
Mouse Il15 Predicted Functions
Enables cytokine activity. Involved in negative regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including T cell activation; lymph node development; and regulation of lymphocyte activation. Located in cytoplasm. Is expressed in several structures, including adrenal gland; genitourinary system; gut; immune system; and nervous system. Human ortholog(s) of this gene implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Orthologous to human IL15 (interleukin 15).
Rat Il15 Target Information
- Target Symbol: Il15, interleukin 15
- Alias:
- IL-15
- interleukin-15
- NCBI_Gene: 25670
- UniProtKB: P97604
Rat Il15 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including cellular response to vitamin D; positive regulation of tyrosine phosphorylation of STAT protein; and skeletal muscle atrophy. Located in cell surface and extracellular space. Used to study pancreatitis. Biomarker of listeriosis and periodontal disease. Human ortholog(s) of this gene implicated in aspergillosis; asthma; pleurisy; and type 1 diabetes mellitus. Orthologous to human IL15 (interleukin 15).
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