Mouse LIF ELISA Kit

$350.00$450.00

TARGETS LIF
SPECIES Mouse
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 50 μL
Sensitivity 0.77 pg/mL
Range 7.81 pg/mL – 500 pg/mL
Assay Time 3.5 h
Recovery 91% – 120%
Average Recovery 1.08
Sample Type Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume 50 μL
Sensitivity 0.77 pg/mL
Range 7.81 pg/mL – 500 pg/mL
Assay Time 3.5 h
Recovery 91% – 120%
Average Recovery 1.08
Intra Precision 3.8% – 4.8%
Inter-Precision 3.9% – 5.9%
Platform ELISA
Plate Detachable 96-well plate
Size 96T/48T
Storage If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery 4℃ blue ice transportation
Components 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-LIF monoclonal antibody
Human LIF freeze-dried standard
Human LIF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay Principle This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse LIF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, LIF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of LIF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

LIF

LIF Target Infomation Overview

  • Target Symbol: LIF, LIF interleukin 6 family cytokine
  • Gene Groups: Receptor ligands; Interleukin 6 type cytokine family
  • Alias: CDF; DIA; HILDA
  • Alias Names: differentiation inhibitory activity; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; human interleukin in DA cells; leukemia inhibitory factor; LIF, interleukin 6 family cytokine

LIF, LIF interleukin 6 family cytokine Target Infomation by Species

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Human LIF Target Information

  • Target Symbol: LIF, LIF interleukin 6 family cytokine
  • Alias:
    • CDF
    • cholinergic differentiation factor
    • D factor
    • DIA
    • differentiation inhibitory activity
    • differentiation stimulating factor
    • differentiation-inducing factor
    • differentiation-stimulating factor
    • hepatocyte-stimulating factor III
    • HILDA
    • human interleukin in DA cells
    • leukemia inhibitory factor
    • LIF, interleukin 6 family cytokine
    • melanoma-derived LPL inhibitor
    • MLPLI
  • NCBI_Gene: 3976
  • UniProtKB: P15018

Human LIF Predicted Functions

Enables cytokine activity; growth factor activity; and leukemia inhibitory factor receptor binding activity. Involved in several processes, including leukemia inhibitory factor signaling pathway; positive regulation of protein modification process; and regulation of epithelial cell differentiation involved in kidney development. Acts upstream of or within several processes, including positive regulation of MAPK cascade; positive regulation of macrophage differentiation; and positive regulation of protein phosphorylation. Located in cytosol.

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Mouse Lif Target Information

Mouse Lif Predicted Functions

Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).

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Rat Lif Target Information

  • Target Symbol: Lif, LIF, interleukin 6 family cytokine
  • Alias:
    • cholinergic neuronal differentiation factor
    • leukemia inhibitory factor
    • leukemia inhibitory factor (cholinergic differentiation factor)
  • NCBI_Gene: 60584

Rat Lif Predicted Functions

Enables leukemia inhibitory factor receptor binding activity. Involved in several processes, including negative regulation of angiogenesis; positive regulation of corticotropin secretion; and transdifferentiation. Located in extracellular space. Biomarker of brain ischemia and congestive heart failure. Orthologous to human LIF (LIF interleukin 6 family cytokine).

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LIF Target News

[catlist tags=”lif” template=targetnews thumbnail=yes thumbnail_class=”related-post-media clr” thumbnail_size=833]

Multiple Sclerosis
Osteoarthrosis & Rheumatoid Arthritis

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