Mouse TSLP ELISA Kit

$350.00$450.00

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TARGETSTSLP
SPECIESMouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.45 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery98% – 119%
Average Recovery1.1
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.45 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery98% – 119%
Average Recovery1.1
Intra Precision2.3% – 3.4%
Inter-Precision3.4% – 7.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TSLP monoclonal antibody
Mouse TSLP freeze-dried standard
TSLP detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse TSLP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TSLP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TSLP in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

TSLP

TSLP Target Infomation Overview

  • Target Symbol: TSLP, thymic stromal lymphopoietin

TSLP, thymic stromal lymphopoietin Target Infomation by Species

HumanMouseRat

Human TSLP Target Information

Human TSLP Predicted Functions

Enables cytokine activity and interleukin-7 receptor binding activity. Involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Located in extracellular region. Biomarker of common cold and respiratory syncytial virus infectious disease.

Mouse Tslp Target Information

Mouse Tslp Predicted Functions

Enables cytokine activity. Predicted to be involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Predicted to be located in extracellular region. Is expressed in ductus deferens; epididymis; ileum; and testis. Used to study atopic dermatitis; autoimmune hemolytic anemia; cryoglobulinemia; and membranoproliferative glomerulonephritis. Orthologous to human TSLP (thymic stromal lymphopoietin).

Rat Tslp Target Information

Rat Tslp Predicted Functions

Predicted to enable cytokine activity and interleukin-7 receptor binding activity. Predicted to be involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Predicted to be located in extracellular region. Orthologous to human TSLP (thymic stromal lymphopoietin).

TSLP Target News

    5.4
    Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice
    JOURNAL OF ETHNOPHARMACOLOGY
    5.4
    CKBA suppresses mast cell activation via ERK signaling pathway in murine atopic dermatitis
    EUROPEAN JOURNAL OF IMMUNOLOGY
    Allergic Reaction
    Lung Disease (Asthma & COPD)

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