Mouse TSLP ELISA Kit
$350.00 – $450.00
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
---|---|
Sample Volume | 20 μL |
Sensitivity | 0.45 pg/mL |
Range | 31.25 pg/mL – 2000 pg/mL |
Assay Time | 3.5 h |
Recovery | 98% – 119% |
Average Recovery | 1.1 |
Intra Precision | 2.3% – 3.4% |
Inter-Precision | 3.4% – 7.4% |
Platform | ELISA |
Plate | Detachable 96-well plate |
Size | 96T/48T |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TSLP monoclonal antibody Mouse TSLP freeze-dried standard TSLP detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse TSLP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, TSLP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of TSLP in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
TSLP
TSLP Target Infomation Overview
- Target Symbol: TSLP, thymic stromal lymphopoietin
TSLP, thymic stromal lymphopoietin Target Infomation by Species
Human TSLP Target Information
- Target Symbol: TSLP, thymic stromal lymphopoietin
- Alias:
- 无
- NCBI_Gene: 85480
- UniProtKB: Q969D9
Human TSLP Predicted Functions
Enables cytokine activity and interleukin-7 receptor binding activity. Involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Located in extracellular region. Biomarker of common cold and respiratory syncytial virus infectious disease.
Mouse Tslp Target Information
- Target Symbol: Tslp, thymic stromal lymphopoietin
- Alias:
- 无
- NCBI_Gene: 53603
Mouse Tslp Predicted Functions
Enables cytokine activity. Predicted to be involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Predicted to be located in extracellular region. Is expressed in ductus deferens; epididymis; ileum; and testis. Used to study atopic dermatitis; autoimmune hemolytic anemia; cryoglobulinemia; and membranoproliferative glomerulonephritis. Orthologous to human TSLP (thymic stromal lymphopoietin).
Rat Tslp Target Information
- Target Symbol: Tslp, thymic stromal lymphopoietin
- Alias:
- LOC688621
- similar to thymic stromal lymphopoietin
- NCBI_Gene: 688621
- UniProtKB: A0A0G2JUJ3
Rat Tslp Predicted Functions
Predicted to enable cytokine activity and interleukin-7 receptor binding activity. Predicted to be involved in several processes, including defense response to other organism; positive regulation of cytokine production; and positive regulation of signal transduction. Predicted to be located in extracellular region. Orthologous to human TSLP (thymic stromal lymphopoietin).