Rat BDNF ELISA Kit

$245.00 – $350.00

Related Target	BDNF
Species	rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.54 pg/mL
Array Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	84% – 118%
Average Recovery	105%

ELISA Kit Detail Information

Related Target	BDNF
Species	rat
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.54 pg/mL
Array Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	84% – 118%
Average Recovery	105%
Intra Precision	2.7% – 4.7%
Inter Precision	3.9% - 4.9%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-BDNF monoclonal antibody Rat BDNF freeze-dried standard BDNF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-rat BDNF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, BDNF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BDNF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

BDNF

No description available.

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