EasyGo!™ Human CRP One-Step ELISA Kit

EK194EG

EasyGo!™ Human CRP One-Step ELISA Kit

$349.00 – $499.00

 DATASHEET
Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity2.42pg/ml
Array Range31.25-2000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
SKU: EK194EG Category:

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100uL(diluent)
Sensitivity2.42pg/ml
Array Range31.25-2000pg/ml
Assay Time90min
Recovery70%-130%
Average Recovery/
Intra Precision< 10%
Inter Precision< 10%
PlateDetachable 96-well Plate
Storage2-8℃
Deliveryblue ice
ComponentsCoated Microplate
standard
Assay Buffer (10×)
TMB
Stop Solution
Washing Buffer (20×)
Assay PrincipleThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader.

Related Targets

CRP

CRP Target Infomation Overview

  • Target Symbol: CRP, C-reactive protein
  • Gene Groups: Short pentraxins
  • Alias: PTX1
  • Alias Names: pentraxin 1; C-reactive protein, pentraxin-related

CRP, C-reactive protein Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human CRP Target Information

  • Target Symbol: CRP, C-reactive protein
  • Alias:
    • C-reactive protein, pentraxin-related
    • c-reactive protein, petaxin related
    • MGC149895
    • MGC88244
    • pentraxin 1
    • PTX1
  • NCBI_Gene: 1401
  • UniProtKB: P02741

Human CRP Predicted Functions

Enables several functions, including complement component C1q complex binding activity; low-density lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including negative regulation of macrophage derived foam cell differentiation; positive regulation of superoxide anion generation; and regulation of gene expression. Acts upstream of or within vasoconstriction. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); autoimmune disease (multiple); eye disease (multiple); and lung disease (multiple).

Mouse Crp Target Information

Mouse Crp Predicted Functions

Predicted to enable several functions, including cholesterol binding activity; complement component C1q complex binding activity; and low-density lipoprotein particle binding activity. Predicted to be involved in several processes, including complement activation, classical pathway; regulation of gene expression; and regulation of superoxide anion generation. Predicted to act upstream of or within vasoconstriction. Predicted to be located in filopodium and growth cone. Predicted to be active in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).

Rat Crp Target Information

Rat Crp Predicted Functions

Enables cholesterol binding activity; identical protein binding activity; and low-density lipoprotein particle binding activity. Involved in several processes, including cellular response to nitric oxide; complement activation, classical pathway; and negative regulation of superoxide anion generation. Located in extracellular space; filopodium; and growth cone. Used to study rheumatoid arthritis. Biomarker of several diseases, including acute kidney failure; artery disease (multiple); iron deficiency anemia; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).

Products with the Same Target - CRP

EasyGo!™ Mouse CRP One-Step ELISA Kit

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Rat C-Reactive Protein/CRP ELISA Kit

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Mouse C-Reactive Protein/CRP ELISA Kit

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Human C-Reactive Protein ELISA Kit

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Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA: Principles, Methods, and Key Differences

Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.

Continue Reading Competitive ELISA: Principles, Methods, and Key Differences

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