EK294

Mouse C-Reactive Protein/CRP ELISA Kit

$245.00$350.00

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity0.27 pg/mL
Array Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery95% – 106%
Average Recovery101%

ELISA Kit Detail Information

Related Target
Speciesmouse
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity0.27 pg/mL
Array Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery95% – 106%
Average Recovery101%
Intra Precision3.9% – 4.8%
Inter Precision3.6% – 4.9%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CRP monoclonal antibody
Mouse CRP freeze-dried standard
CRP detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse CRP antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CRP present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CRP in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CRP

CRP Target Infomation Overview

  • Target Symbol: CRP, C-reactive protein
  • Gene Groups: Short pentraxins
  • Alias: PTX1
  • Alias Names: pentraxin 1; C-reactive protein, pentraxin-related

CRP, C-reactive protein Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human CRP Target Information

  • Target Symbol: CRP, C-reactive protein
  • Alias:
    • C-reactive protein, pentraxin-related
    • c-reactive protein, petaxin related
    • MGC149895
    • MGC88244
    • pentraxin 1
    • PTX1
  • NCBI_Gene: 1401
  • UniProtKB: P02741

Human CRP Predicted Functions

Enables several functions, including complement component C1q complex binding activity; low-density lipoprotein particle binding activity; and low-density lipoprotein particle receptor binding activity. Involved in several processes, including negative regulation of macrophage derived foam cell differentiation; positive regulation of superoxide anion generation; and regulation of gene expression. Acts upstream of or within vasoconstriction. Located in extracellular space. Implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Biomarker of several diseases, including artery disease (multiple); auditory system disease (multiple); autoimmune disease (multiple); eye disease (multiple); and lung disease (multiple).

Mouse Crp Target Information

Mouse Crp Predicted Functions

Predicted to enable several functions, including cholesterol binding activity; complement component C1q complex binding activity; and low-density lipoprotein particle binding activity. Predicted to be involved in several processes, including complement activation, classical pathway; regulation of gene expression; and regulation of superoxide anion generation. Predicted to act upstream of or within vasoconstriction. Predicted to be located in filopodium and growth cone. Predicted to be active in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).

Rat Crp Target Information

Rat Crp Predicted Functions

Enables cholesterol binding activity; identical protein binding activity; and low-density lipoprotein particle binding activity. Involved in several processes, including cellular response to nitric oxide; complement activation, classical pathway; and negative regulation of superoxide anion generation. Located in extracellular space; filopodium; and growth cone. Used to study rheumatoid arthritis. Biomarker of several diseases, including acute kidney failure; artery disease (multiple); iron deficiency anemia; obesity; and status epilepticus. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); macular degeneration (multiple); middle cerebral artery infarction; and nephritis (multiple). Orthologous to human CRP (C-reactive protein).

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