Powered by BiozEasyGo!™ Mouse CD34 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10uL |
| Sensitivity | 0.08 ng/ml |
| Array Range | 0.16-10ng/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CD34
CD34 Target Infomation Overview
- Target Symbol: CD34, CD34 molecule
- Gene Groups: CD molecules
- Alias: CD34 antigen
- Alias Names: Hematopoietic progenitor cell antigen CD34, CD34 sialomucin
CD34, CD34 molecule Target Infomation by Species
- Human
- 小鼠 Mouse
- Rat
人 Human CD34 Target Information
- Target Symbol: CD34, CD34 molecule
- Alias:
- CD34 antigen
- hematopoietic progenitor cell antigen CD34
- NCBI_Gene: 947
- UniProtKB: P28906
Human CD34 Predicted Functions
Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and carbohydrate binding activity. Involved in several processes, including blood vessel development; regulation of cellular response to stress; and regulation of cytokine production. Located in external side of plasma membrane; intercellular bridge; and perinuclear region of cytoplasm. Biomarker of thyroid gland carcinoma.
Mouse Cd34 Target Information
- Target Symbol: Cd34, CD34 antigen
- Alias:
- AU040960
- expressed sequence AU040960
- NCBI_Gene: 12490
Mouse Cd34 Predicted Functions
Enables sulfate binding activity. Acts upstream of or within leukocyte migration. Located in cytoplasm; external side of plasma membrane; and extracellular region. Is expressed in several structures, including alimentary system; cardiovascular system; extraembryonic component; genitourinary system; and skin. Orthologous to human CD34 (CD34 molecule).
Rat Cd34 Target Information
- Target Symbol: Cd34, CD34 molecule
- Alias:
- CD34 antigen
- hematopoietic progenitor cell antigen CD34
- LOC305081
- NCBI_Gene: 305081
Rat Cd34 Predicted Functions
Predicted to enable RNA polymerase II-specific DNA-binding transcription factor binding activity; carbohydrate binding activity; and sulfate binding activity. Involved in glomerular filtration and mesangial cell-matrix adhesion. Located in apical plasma membrane; basal plasma membrane; and lysosome. Part of glomerular endothelium fenestra. Biomarker of diabetic retinopathy. Orthologous to human CD34 (CD34 molecule).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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