Powered by BiozEasyGo!™ Mouse CD5L One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 100uL(diluent) |
| Sensitivity | 4.46 pg/mL |
| Array Range | 46.88-3000pg/mL |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
CD5L
CD5L Target Infomation Overview
- Target Symbol: CD5L, CD5 molecule like
- Gene Groups: Scavenger receptor cysteine rich domain containing
- Alias: Spalpha, API6
- Alias Names: apoptosis inhibitor 6; CD5 antigen-like (scavenger receptor cysteine rich family); CD5 molecule-like
CD5L, CD5 molecule like Target Infomation by Species
- Human
- Mouse
- Rat
Human CD5L Target Information
- Target Symbol: CD5L, CD5 molecule like
- Alias:
- AIM
- API6
- apoptosis inhibitor 6
- apoptosis inhibitor expressed by macrophages
- apoptosis inhibitor of macrophage
- CD5 antigen-like
- CD5 antigen-like (scavenger receptor cysteine rich family)
- CD5 molecule-like
- CT-2
- hAIM
- igM-associated peptide
- PRO229
- SP-ALPHA
- Spalpha
- NCBI_Gene: 922
- UniProtKB: P43866
Human CD5L Predicted Functions
Predicted to be involved in cellular defense response. Predicted to act upstream of or within positive regulation of complement-dependent cytotoxicity and regulation of complement activation. Located in cell surface.
Mouse Cd5l Target Information
- Target Symbol: Cd5l, CD5 antigen-like
- Alias:
- AAC-11
- AI047839
- AIM
- AIM/Spalpha
- Api6
- apoptosis inhibitor 6
- expressed sequence AI047839
- Pdp 1/6
- Sp-alpha
- NCBI_Gene: 11801
Mouse Cd5l Predicted Functions
Acts upstream of or within positive regulation of complement-dependent cytotoxicity and regulation of complement activation. Located in cell surface and plasma membrane. Is expressed in several structures, including central nervous system; immune system; liver; retina; and testis. Orthologous to human CD5L (CD5 molecule like).
Rat Cd5l Target Information
- Target Symbol: Cd5l, CD5 antigen-like
- Alias:
- CD5 antigen-like
- LOC310693
- MGC112716
- NCBI_Gene: 310693
Rat Cd5l Predicted Functions
Predicted to act upstream of or within positive regulation of complement-dependent cytotoxicity and regulation of complement activation. Predicted to be located in cell surface. Predicted to be active in plasma membrane. Orthologous to human CD5L (CD5 molecule like).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
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