Powered by BiozEasyGo!™ Mouse EpCAM/CD326 One-Step ELISA Kit
$349.00 – $499.00
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 10uL |
| Sensitivity | 38.29 pg/ml |
| Array Range | 78.13-5000pg/ml |
| Assay Time | 90min |
| Recovery | 70%-130% |
| Average Recovery | / |
| Intra Precision | < 10% |
| Inter Precision | < 10% |
| Plate | Detachable 96-well Plate |
| Storage | 2-8℃ |
| Delivery | blue ice |
| Components | Coated Microplate standard Assay Buffer (10×) TMB Stop Solution Washing Buffer (20×) |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IL-18 has been immobilized onto microwells, and one pellet of the HRP-linked detect antibody specific for IL-18 (light yellow) is pre-placed in the microwells, sealed by the adhesive film. Standard or samples are pipetted into the wells, then IL-18 present is bound by the immobilized antibody and detect antibody in the incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-18 bound by the immobilized antibody. The color development is stopped and the intensity of the color is measured by microplate reader. |
Related Targets
EPCAM
EPCAM Target Infomation Overview
- Target Symbol: EPCAM, epithelial cell adhesion molecule
- Gene Groups: CD molecules; MicroRNA protein coding host genes
- Alias: Ly74; TROP1; GA733-2; EGP34; EGP40; EGP-2; KSA; CD326; Ep-CAM; HEA125; KS1/4; MK-1; MH99; MOC31; MOC-31; 323/A3; 17-1A; TACST-1; CO-17A; ESA; BerEp4; Ber-Ep4; M4S1; MIC18; TACSTD1
- Alias Names: antigen identified by monoclonal antibody AUA1; tumor-associated calcium signal transducer 1; trophoblast cell surface antigen 1
EPCAM, epithelial cell adhesion molecule Target Infomation by Species
- Human
- Mouse
- Rat
Human EPCAM Target Information
- Target Symbol: EPCAM, epithelial cell adhesion molecule
- Alias:
- adenocarcinoma-associated antigen
- Ber-Ep4
- BerEp4
- cell surface glycoprotein Trop-1
- CO-17A
- CO17-1A
- DIAR5
- EGP
- EGP-2
- EGP314
- EGP34
- EGP40
- Ep-CAM
- epithelial glycoprotein 314
- ESA
- GA733-2
- hEGP-2
- HNPCC8
- human epithelial glycoprotein-2
- KS1/4
- KSA
- LYNCH8
- M4S1
- major gastrointestinal tumor-associated protein GA733-2
- membrane component, chromosome 4, surface marker (35kD glycoprotein)
- MIC18
- MK-1
- MOC-31
- TACST-1
- TACSTD1
- TROP1
- trophoblast cell surface antigen 1
- tumor-associated calcium signal transducer 1
- NCBI_Gene: 4072
- UniProtKB: P16422
Human EPCAM Predicted Functions
Predicted to enable cadherin binding activity involved in cell-cell adhesion. Involved in several processes, including negative regulation of cell-cell adhesion mediated by cadherin; positive regulation of stem cell proliferation; and regulation of gene expression. Located in several cellular components, including basolateral plasma membrane; bicellular tight junction; and lateral plasma membrane. Implicated in congenital diarrhea 5 with tufting enteropathy and hereditary nonpolyposis colorectal cancer type 8. Biomarker of hepatocellular carcinoma.
Mouse Epcam Target Information
- Target Symbol: Epcam, epithelial cell adhesion molecule
- Alias:
- CD326
- EGP-2
- Egp314
- Ep-CAM
- EpCAM
- EpCAM1
- epithelial glycoprotein 314
- GA733-2
- gp40
- Ly74
- lymphocyte antigen 74
- panepithelial glycoprotein 314
- Tacstd1
- TROP1
- tumor-associated calcium signal transducer 1
- NCBI_Gene: 17075
Mouse Epcam Predicted Functions
Predicted to enable cadherin binding activity involved in cell-cell adhesion. Involved in ureteric bud development. Located in cell surface. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Used to study congenital diarrhea 5 with tufting enteropathy. Human ortholog(s) of this gene implicated in congenital diarrhea 5 with tufting enteropathy and hereditary nonpolyposis colorectal cancer type 8. Orthologous to human EPCAM (epithelial cell adhesion molecule).
Rat Epcam Target Information
- Target Symbol: Epcam, epithelial cell adhesion molecule
- Alias:
- Egp314
- ep-CAM
- epithelial glycoprotein 314
- Tacstd1
- tumor-associated calcium signal transducer 1
- NCBI_Gene: 171577
Rat Epcam Predicted Functions
Enables cadherin binding activity involved in cell-cell adhesion. Involved in cell-cell adhesion via plasma-membrane adhesion molecules; negative regulation of apoptotic process; and positive regulation of cell motility. Located in bicellular tight junction; cell surface; and lateral plasma membrane. Biomarker of middle cerebral artery infarction. Human ortholog(s) of this gene implicated in congenital diarrhea 5 with tufting enteropathy and hereditary nonpolyposis colorectal cancer type 8. Orthologous to human EPCAM (epithelial cell adhesion molecule).
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
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