Human Amphiregulin ELISA Kit

$299.00 – $419.00

Related Target	AREG
Species	human
Sample Type	Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	2.68 pg/mL
Array Range	7.81 pg/mL - 500 pg/mL
Assay Time	3.5 h
Recovery	88% - 117%
Average Recovery	97%

SKU: EK1378 Category: ELISA Kit

ELISA Kit Detail Information

Related Target	AREG
Species	human
Sample Type	Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples
Sample Volume	Serum, plasma: 20 μL, cell culture supernatant: 100 μL
Sensitivity	2.68 pg/mL
Array Range	7.81 pg/mL - 500 pg/mL
Assay Time	3.5 h
Recovery	88% - 117%
Average Recovery	97%
Intra Precision	2.6% - 3.6%
Inter Precision	2.4% - 5.4%
Plate	Detachable 96-well Plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ Blue Ice Transport
Components	1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Detect Antibody Standard Diluent Streptavidin-HRP Standard Microplate Wells
Assay Principle	This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Amphiregulin has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Amphiregulin are pipetted into the wells and Amphiregulin present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Related Targets

AREG

AREG Target Infomation Overview

Target Symbol: AREG, amphiregulin
Gene Groups: Receptor ligands
Alias: SDGF, AREGB, AR, CRDGF
Alias Names: schwannoma-derived growth factor, amphiregulin B, Colorectum Cell-Derived Growth Factor

AREG, amphiregulin Target Infomation by Species

Human
Mouse
Rat

Human AREG Target Infomation

Target Symbol: AREG, amphiregulin
Alias:
- amphiregulin B
- AR
- AREGB
- colorectum cell-derived growth factor
- CRDGF
- MGC13647
- schwannoma-derived growth factor
- SDGF
NCBI_Gene: 374
UniProtKB: P15514

Human AREG Predicted Functions

Enables receptor ligand activity and transmembrane receptor protein tyrosine kinase activator activity. Involved in ERBB2-EGFR signaling pathway; G protein-coupled receptor signaling pathway; and positive regulation of cell population proliferation. Located in cell surface. Is active in extracellular space. Implicated in breast cancer. Biomarker of ductal carcinoma in situ; invasive ductal carcinoma; prostate carcinoma in situ; transitional cell carcinoma; and urinary bladder cancer.

Mouse Areg Target Information

Target Symbol: Areg, amphiregulin
Alias:
- AR
- Mcub
- modifier of curly bare
- schwannoma derived growth factor
- Sdgf
NCBI_Gene: 11839
- UniprotKB: P31955
- UniprotKB: Q4FJT2

Mouse Areg Predicted Functions

Enables receptor ligand activity and transmembrane receptor protein tyrosine kinase activator activity. Involved in epidermal growth factor receptor signaling pathway. Acts upstream of or within several processes, including mammary gland development; positive regulation of peptidyl-tyrosine phosphorylation; and response to estradiol. Located in cytoplasm and nucleus. Is active in extracellular space. Is expressed in several structures, including alimentary system; heart; lung; metanephros; and submandibular gland primordium. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to human AREG (amphiregulin).

Rat Areg Target Information

Target Symbol: Areg, angiotensinogen
Alias:
- AR
- schwannoma-derived growth factor
- SDGF
NCBI_Gene: 29183
UniProtKB: P24338

Rat Areg Predicted Functions

Enables epidermal growth factor receptor binding activity and growth factor activity. Involved in several processes, including negative regulation of osteoblast differentiation; neurogenesis; and response to hydrogen peroxide. Located in extracellular space. Used to study high grade glioma. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to human AREG (amphiregulin).

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