Human Angiotensinogen/AGT/ SerpinA8 ELISA Kit

$350.00$450.00

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TARGETSAGT
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity19.93 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery83% – 105%
Average Recovery0.96
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity19.93 pg/mL
Range0.31 ng/mL – 20 ng/mL
Assay Time3.5 h
Recovery83% – 105%
Average Recovery0.96
Intra Precision4.0% – 4.9%
Inter-Precision3.7%-4.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Angiotensinogen/AGT/ SerpinA8 monoclonal antibody
Human Angiotensinogen/AGT/ SerpinA8 freeze-dried standard
Angiotensinogen/AGT/ SerpinA8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human AGT antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, AGT present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of AGT in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

AGT

AGT Target Infomation Overview

  • Target Symbol: AGT, angiotensinogen
  • Gene Groups: Serpin peptidase inhibitors; Neuropeptides
  • Previous Names: SERPINA8
  • Alias Names: alpha-1 antiproteinase, antitrypsin; serpin peptidase inhibitor, clade A, member 8

AGT, angiotensinogen Target Infomation by Species

HumanMouseRat

Human AGT Target Information

  • Target Symbol: AGT, angiotensinogen
  • Alias:
    • alpha-1 antiproteinase, antitrypsin
    • angiotensin I
    • angiotensin II
    • angiotensinogen (serine (or cysteine) proteinase inhibitor, clade a (alpha-1 antiproteinase, antitrypsin), member 8)
    • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
    • ANHU
    • fetal-liver predominant transporter 1
    • FLJ92595
    • FLJ97926
    • hFLT1
    • pre-angiotensinogen
    • serine (or cysteine) proteinase inhibitor
    • serpin A8
    • serpin peptidase inhibitor, clade A, member 8
    • SERPINA8
  • NCBI_Gene: 183
  • UniProtKB: P01019

Human AGT Predicted Functions

Enables angiotensin receptor binding activity; receptor ligand activity; and sodium channel regulator activity. Involved in several processes, including positive regulation of peptidyl-tyrosine phosphorylation; regulation of gene expression; and regulation of signal transduction. Located in extracellular space. Part of collagen-containing extracellular matrix. Implicated in several diseases, including Fabry disease; Henoch-Schoenlein purpura; artery disease (multiple); heart conduction disease (multiple); and hydronephrosis. Biomarker of several diseases, including alcoholic hepatitis; chronic myeloid leukemia; intermediate coronary syndrome; systemic scleroderma (multiple); and type 2 diabetes mellitus.

Mouse Agt Target Information

Mouse Agt Predicted Functions

Enables type 1 angiotensin receptor binding activity and type 2 angiotensin receptor binding activity. Involved in positive regulation of cytokine production. Acts upstream of or within several processes, including nervous system development; positive regulation of protein phosphorylation; and regulation of systemic arterial blood pressure by renin-angiotensin. Located in extracellular space. Is expressed in several structures, including axial skeleton; central nervous system; foregut mesenchyme; genitourinary system; and liver. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Henoch-Schoenlein purpura; artery disease (multiple); heart conduction disease (multiple); and hydronephrosis. Orthologous to human AGT (angiotensinogen).

Rat Agt Target Information

  • Target Symbol: Agt, angiotensinogen
  • Alias:
    • Ang
    • AngII
    • angiotensin II
    • angiotensinogen (PAT)
    • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
    • ANRT
    • MGC105326
    • PAT
    • serpin A8
  • NCBI_Gene: 24179
  • UniProtKB: P01015

Rat Agt Predicted Functions

Enables hormone activity. Involved in several processes, including MAPK cascade; regulation of ion transport; and regulation of secretion. Located in cytoplasm and extracellular space. Used to study several diseases, including artery disease (multiple); diabetic retinopathy; gastric ulcer; impotence; and sciatic neuropathy. Biomarker of several diseases, including cerebrovascular disease (multiple); glomerulonephritis (multiple); liver cirrhosis (multiple); lung disease (multiple); and prediabetes syndrome. Human ortholog(s) of this gene implicated in several diseases, including Fabry disease; Henoch-Schoenlein purpura; artery disease (multiple); heart conduction disease (multiple); and hydronephrosis. Orthologous to human AGT (angiotensinogen).

AGT Target News

    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Obesity Diabetes

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