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Human Apolipoprotein A1/Apo A1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 90 μL 1×Assay Buffer and 10 μL prediluted sample |
| Sensitivity | 0.73 ng/mL |
| Range | 3.31 ng/mL – 200 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 90% – 110% |
| Average Recovery | 1.01 |
| Intra Precision | 3.4% – 6.9% |
| Inter-Precision | 2.0%-6.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Apolipoprotein A1/Apo A1 monoclonal antibody Human Apolipoprotein A1/Apo A1 freeze-dried standard Apolipoprotein A1/Apo A1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Apo A1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Apo A1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Apo A1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
APOA1
APOA1 Target Infomation Overview
- Target Symbol: APOA1, apolipoprotein A1
- Gene Groups: Apolipoproteins
- Alias Names: apolipoprotein A-I
APOA1, apolipoprotein A1 Target Infomation by Species
HumanMouseRat
Human APOA1 Target Information
- Target Symbol: APOA1, apolipoprotein A1
- Alias:
- apo-AI
- apo(a)
- apoA-I
- apolipoprotein A-I
- HPALP2
- MGC117399
- NCBI_Gene: 335
- UniProtKB: P02647
Human APOA1 Predicted Functions
Enables several functions, including amyloid-beta binding activity; cholesterol binding activity; and signaling receptor binding activity. Contributes to cholesterol transfer activity. Involved in several processes, including lipid homeostasis; lipid transport; and regulation of Rho protein signal transduction. Acts upstream of or within cholesterol transport. Located in endocytic vesicle and extracellular space. Part of spherical high-density lipoprotein particle and very-low-density lipoprotein particle. Implicated in several diseases, including artery disease (multiple); familial visceral amyloidosis; hypolipoproteinemia (multiple); non-alcoholic steatohepatitis; and systemic lupus erythematosus. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); end stage renal disease; liver disease (multiple); and neurodegenerative disease (multiple).
Mouse Apoa1 Target Information
- Target Symbol: Apoa1, apolipoprotein A-I
- Alias:
- Alp-1
- Apoa-1
- brain protein 14
- Brp-14
- liver 20-30 thousand M.Wt protein 1
- Ltw-1
- Lvtw-1
- Sep-1
- Sep-2
- Sep2
- serum protein 1
- serum protein 2
- NCBI_Gene: 11806
Mouse Apoa1 Predicted Functions
Enables several functions, including cholesterol transfer activity; high-density lipoprotein particle binding activity; and identical protein binding activity. Acts upstream of or within several processes, including cholesterol biosynthetic process; cholesterol efflux; and regulation of intestinal cholesterol absorption. Located in extracellular space. Is expressed in several structures, including adrenal gland; gonad; gut; liver; and lung. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); familial visceral amyloidosis; hypolipoproteinemia (multiple); non-alcoholic steatohepatitis; and systemic lupus erythematosus. Orthologous to human APOA1 (apolipoprotein A1).
Rat Apoa1 Target Information
- Target Symbol: Apoa1, apolipoprotein A1
- Alias:
- apo-AI
- apoA-I
- apolipoprotein A-1
- apolipoprotein A-I
- preproapolipoprotein A-I
- NCBI_Gene: 25081
- UniProtKB: P04639
Rat Apoa1 Predicted Functions
Enables amyloid-beta binding activity; cholesterol transfer activity; and lipase inhibitor activity. Involved in several processes, including lipid transport; peripheral nervous system axon regeneration; and response to estrogen. Located in cell surface; extracellular space; and nucleus. Part of discoidal high-density lipoprotein particle; intermediate-density lipoprotein particle; and low-density lipoprotein particle. Used to study arteriosclerosis and hyperthyroidism. Biomarker of several diseases, including fatty liver disease (multiple); hypertension; major depressive disorder; ocular hypertension; and sciatic neuropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); familial visceral amyloidosis; hypolipoproteinemia (multiple); non-alcoholic steatohepatitis; and systemic lupus erythematosus. Orthologous to human APOA1 (apolipoprotein A1).
