Human Arginase 1/ARG1 ELISA Kit

$350.00$450.00

Download:
TARGETSARG1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity26.80 pg/mL
Range0.78 ng/mL – 50 ng/mL
Assay Time3.5 h
Recovery75% – 124%
Average Recovery1.02
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity26.80 pg/mL
Range0.78 ng/mL – 50 ng/mL
Assay Time3.5 h
Recovery75% – 124%
Average Recovery1.02
Intra Precision3.4% – 4.6%
Inter-Precision2.6% – 4.8%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-ARG1 monoclonal antibody
Human ARG1 freeze-dried standard
Angiotensinogen/AGT/ SerpinA8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human ARG1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, ARG1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of ARG1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

ARG1

ARG1 Target Infomation Overview

  • Target Symbol: ARG1, arginase 1
  • Alias Names: arginase-1; arginase, liver

ARG1, arginase 1 Target Infomation by Species

HumanMouseRat

Human ARG1 Target Information

Human ARG1 Predicted Functions

Predicted to enable arginase activity and manganese ion binding activity. Involved in negative regulation of T cell proliferation; negative regulation of interferon-gamma-mediated signaling pathway; and positive regulation of neutrophil mediated killing of fungus. Located in extracellular space. Implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Biomarker of asthma and colitis.

Mouse Arg1 Target Information

Mouse Arg1 Predicted Functions

Enables arginase activity. Involved in defense response to protozoan; negative regulation of T-helper 2 cell cytokine production; and negative regulation of activated T cell proliferation. Predicted to be located in several cellular components, including extracellular space; mitochondrial outer membrane; and neuronal cell body. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Used to study hyperargininemia. Human ortholog(s) of this gene implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Orthologous to human ARG1 (arginase 1).

Rat Arg1 Target Information

  • Target Symbol: Arg1, arginase 1
  • Alias:
    • AI type I arginase
    • AI, type I arginase
    • arginase 1 liver
    • Arginase 1, liver
    • arginase-1
    • arginase, liver
    • liver-type arginase
    • type I arginase
  • NCBI_Gene: 29221
  • UniProtKB: P07824

Rat Arg1 Predicted Functions

Enables arginase activity; identical protein binding activity; and manganese ion binding activity. Involved in several processes, including cellular response to hormone stimulus; gland development; and response to vitamin. Located in several cellular components, including extracellular space; mitochondrial outer membrane; and neuronal cell body. Used to study acute necrotizing pancreatitis and hypertension. Biomarker of several diseases, including acute kidney failure; cholestasis; silicosis; ureteral obstruction; and uveitis. Human ortholog(s) of this gene implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Orthologous to human ARG1 (arginase 1).

ARG1 Target News

    5.139
    Potentiated effects of lactate receptor GPR81 on immune microenvironment in breast cancer
    MOLECULAR CARCINOGENESIS

    Related Products

    more +

    EK1392

    $350.00$450.00

    EK1341

    $350.00$450.00

    EK1C01

    $350.00$450.00

    Same Target