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Human B7-H1/PD-L1/CD274 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 5.58 pg/mL |
| Range | 0.23 ng/mL – 15 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 81% – 104% |
| Average Recovery | 0.85 |
| Intra Precision | 3.4%-5.8% |
| Inter-Precision | 1.3% – 4.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B7-H1 monoclonal antibody Human B7-H1 freeze-dried standard B7-H1 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human B7-H1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, B7-H1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of B7-H1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CD274
CD274 Target Infomation Overview
- Target Symbol: CD274, CD274 molecule
- Gene Groups: CD molecules; Receptor ligands; V-set domain containing; C2-set domain containing; B7 family
- Alias: B7-H; B7H1; PD-L1; PDL1; B7-H1
- Previous Names: PDCD1LG1
- Alias Names: B7 homolog 1; programmed cell death 1 ligand 1; CD274 antigen
CD274, CD274 molecule Target Infomation by Species
HumanMouseRat
Human CD274 Target Information
- Target Symbol: CD274, CD274 molecule
- Alias:
- B7 homolog 1
- B7-H
- B7H1
- CD274 antigen
- hPD-L1
- MGC142294
- MGC142296
- PD-L1
- PDCD1 ligand 1
- PDCD1L1
- PDCD1LG1
- PDL1
- programmed cell death 1 ligand 1
- programmed death ligand 1
- NCBI_Gene: 29126
- UniProtKB: Q9NZQ7
Human CD274 Predicted Functions
Involved in several processes, including positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process; regulation of T cell activation; and regulation of cytokine production. Located in several cellular components, including endosome membrane; extracellular exosome; and nucleoplasm. Biomarker of several diseases, including Cryptococcal meningitis; Human papillomavirus infectious disease; gastrointestinal system cancer (multiple); lymphoma (multiple); and tuberculosis (multiple).
Mouse Cd274 Target Information
- Target Symbol: Cd274, CD274 antigen
- Alias:
- B7-H1
- PD-L1
- Pdcd1lg1
- programmed cell death 1 ligand 1
- NCBI_Gene: 60533
Mouse Cd274 Predicted Functions
Acts upstream of or within negative regulation of T cell proliferation and toxin transport. Located in external side of plasma membrane. Is expressed in retina and thymus primordium. Orthologous to human CD274 (CD274 molecule).
Rat Cd274 Target Information
- Target Symbol: Cd274, CD274 molecule
- Alias:
- CD274 antigen
- LOC499342
- Pdcd1lg1
- programmed cell death 1 ligand 1
- RGD1566211
- similar to PD-1-ligand precursor
- NCBI_Gene: 499342
- UniProtKB: D4AE25
Rat Cd274 Predicted Functions
Involved in positive regulation of T cell proliferation and positive regulation of cell migration. Located in cell surface. Biomarker of periodontitis and pre-eclampsia. Orthologous to human CD274 (CD274 molecule).
