Human B7-H1/PD-L1/CD274 ELISA Kit

$350.00$450.00

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TARGETSCD274
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity5.58 pg/mL
Range0.23 ng/mL – 15 ng/mL
Assay Time3.5 h
Recovery81% – 104%
Average Recovery0.85
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity5.58 pg/mL
Range0.23 ng/mL – 15 ng/mL
Assay Time3.5 h
Recovery81% – 104%
Average Recovery0.85
Intra Precision3.4%-5.8%
Inter-Precision1.3% – 4.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-B7-H1 monoclonal antibody
Human B7-H1 freeze-dried standard
B7-H1 detect Antibody
Standard Diluent
HRP-labeled streptavidin
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human B7-H1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, B7-H1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of B7-H1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CD274

CD274 Target Infomation Overview

  • Target Symbol: CD274, CD274 molecule
  • Gene Groups: CD molecules; Receptor ligands; V-set domain containing; C2-set domain containing; B7 family
  • Alias: B7-H; B7H1; PD-L1; PDL1; B7-H1
  • Previous Names: PDCD1LG1
  • Alias Names: B7 homolog 1; programmed cell death 1 ligand 1; CD274 antigen

CD274, CD274 molecule Target Infomation by Species

HumanMouseRat

Human CD274 Target Information

  • Target Symbol: CD274, CD274 molecule
  • Alias:
    • B7 homolog 1
    • B7-H
    • B7H1
    • CD274 antigen
    • hPD-L1
    • MGC142294
    • MGC142296
    • PD-L1
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL1
    • programmed cell death 1 ligand 1
    • programmed death ligand 1
  • NCBI_Gene: 29126
  • UniProtKB: Q9NZQ7

Human CD274 Predicted Functions

Involved in several processes, including positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process; regulation of T cell activation; and regulation of cytokine production. Located in several cellular components, including endosome membrane; extracellular exosome; and nucleoplasm. Biomarker of several diseases, including Cryptococcal meningitis; Human papillomavirus infectious disease; gastrointestinal system cancer (multiple); lymphoma (multiple); and tuberculosis (multiple).

Mouse Cd274 Target Information

Mouse Cd274 Predicted Functions

Acts upstream of or within negative regulation of T cell proliferation and toxin transport. Located in external side of plasma membrane. Is expressed in retina and thymus primordium. Orthologous to human CD274 (CD274 molecule).

Rat Cd274 Target Information

  • Target Symbol: Cd274, CD274 molecule
  • Alias:
    • CD274 antigen
    • LOC499342
    • Pdcd1lg1
    • programmed cell death 1 ligand 1
    • RGD1566211
    • similar to PD-1-ligand precursor
  • NCBI_Gene: 499342
  • UniProtKB: D4AE25

Rat Cd274 Predicted Functions

Involved in positive regulation of T cell proliferation and positive regulation of cell migration. Located in cell surface. Biomarker of periodontitis and pre-eclampsia. Orthologous to human CD274 (CD274 molecule).

CD274 Target News

    3.253
    Homeobox D9 drives the malignant phenotypes and enhances the Programmed death ligand-1 expression in non-small cell lung cancer cells via binding to Angiopoietin-2 promoter
    World Journal of Surgical Oncology
    Multiple Sclerosis
    Sepsis
    Systemic Lupus Erythematosus
    Transplantation
    Tumor Marker

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