Human BAFF/BLys/TNFSF13B ELISA Kit

ELISA Kits

$350.00 – $450.00

Download:

Datasheet

SDS File

TARGETS	TNFSF13B
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.23 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 121%
Average Recovery	1.02

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	20 μL
Sensitivity	0.23 pg/mL
Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	80% – 121%
Average Recovery	1.02
Intra Precision	3.6%-5.7%
Inter-Precision	3.1% – 5.9%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-BAFF/BLys/TNFSF13B monoclonal antibody Human BAFF/BLys/TNFSF13B freeze-dried standard BAFF/BLys/TNFSF13B detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BAFF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the BAFF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BAFF in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

TNFSF13B

TNFSF13B Target Infomation Overview

Target Symbol: TNFSF13B, TNF superfamily member 13b
Gene Groups: CD molecules; Tumor necrosis factor superfamily
Alias: BAFF; THANK; BLYS; TALL-1; TALL1; CD257
Previous Names: TNFSF20
Alias Names: B-cell-activating factor; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; B-lymphocyte stimulator; tumor necrosis factor (ligand) superfamily, member 13b

TNFSF13B, TNF superfamily member 13b Target Infomation by Species

HumanMouseRat

Human TNFSF13B Target Information

Target Symbol: TNFSF13B, TNF superfamily member 13b
Alias:
- ApoL related ligand TALL-1
- b lymphocyte stimulator
- B-cell activating factor
- B-cell-activating factor
- B-lymphocyte stimulator
- BAFF
- BLYS
- CD257
- delta BAFF
- Delta4 BAFF
- dendritic cell-derived TNF-like molecule
- DTL
- TALL-1
- TALL1
- THANK
- TNF and ApoL-related leukocyte expressed ligand 1
- TNF homolog that activates apoptosis
- TNF- and APOL-related leukocyte expressed ligand 1
- TNFSF20
- TNLG7A
- tumor necrosis factor (ligand) superfamily member 13b
- tumor necrosis factor (ligand) superfamily, member 13b
- tumor necrosis factor (ligand) superfamily, member 20
- tumor necrosis factor ligand 7A
- tumor necrosis factor ligand superfamily member 13B
- tumor necrosis factor superfamily member 13b
- tumor necrosis factor superfamily, member 13b
- tumor necrosis factor-like protein ZTNF4
- ZTNF4
NCBI_Gene: 10673
UniProtKB: Q9Y275

Human TNFSF13B Predicted Functions

Predicted to enable receptor ligand activity. Involved in positive regulation of B cell proliferation. Located in several cellular components, including focal adhesion; perinuclear region of cytoplasm; and plasma membrane.

Mouse Tnfsf13b Target Information

Target Symbol: Tnfsf13b, tumor necrosis factor (ligand) superfamily, member 13b
Alias:
- BAFF
- BLyS
- D8Ertd387e
- DNA segment, Chr 8, ERATO Doi 387, expressed
- TALL-1
- zTNF4
NCBI_Gene: 24099

Mouse Tnfsf13b Predicted Functions

Predicted to enable receptor ligand activity. Involved in B cell differentiation and tumor necrosis factor-mediated signaling pathway. Acts upstream of or within B cell homeostasis; positive regulation of germinal center formation; and positive regulation of lymphocyte activation. Predicted to be located in several cellular components, including focal adhesion; perinuclear region of cytoplasm; and plasma membrane. Predicted to be active in extracellular space. Is expressed in genitourinary system. Used to study IgA glomerulonephritis and Sjogren’s syndrome. Orthologous to human TNFSF13B (TNF superfamily member 13b).

Rat Tnfsf13b Target Information

Target Symbol: Tnfsf13b, TNF superfamily member 13b
Alias:
- B-cell-activating factor
- BAFF
- LOC498666
- RGD1561519
- similar to NZB B-cell activating factor
- Tnlg7a
- tumor necrosis factor (ligand) superfamily, member 13b
- tumor necrosis factor ligand 7a
- tumor necrosis factor ligand superfamily member 13B
- tumor necrosis factor superfamily member 13b
NCBI_Gene: 498666

Rat Tnfsf13b Predicted Functions

Predicted to enable receptor ligand activity. Predicted to be involved in B cell differentiation; positive regulation of B cell proliferation; and tumor necrosis factor-mediated signaling pathway. Predicted to act upstream of or within B cell homeostasis; positive regulation of germinal center formation; and positive regulation of lymphocyte activation. Predicted to be located in several cellular components, including focal adhesion; perinuclear region of cytoplasm; and plasma membrane. Predicted to be active in extracellular space. Orthologous to human TNFSF13B (TNF superfamily member 13b).