Human BID ELISA Kit

$245.00 – $350.00

Related Target	BID
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	5 μL
Sensitivity	0.51 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	93% – 109%
Average Recovery	102%

ELISA Kit Detail Information

Related Target	BID
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	5 μL
Sensitivity	0.51 pg/mL
Array Range	15.63 pg/mL – 1000 pg/mL
Assay Time	3.5 h
Recovery	93% – 109%
Average Recovery	102%
Intra Precision	2.3% – 3.0%
Inter Precision	1.7% – 2.2%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with specific anti-human BID antibody Human BID freeze-dried standard Streptavidin-HRP labeled human BID detect Antibody Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film Standard Diluent
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BID antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, BID present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of BID in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

BID

BID Target Infomation Overview

Target Symbol: BID, BH3 interacting domain death agonist
Gene Groups: Receptor ligands; BCL2 homology region 3 (BH3) only; MicroRNA protein coding host genes

BID, BH3 interacting domain death agonist Target Infomation by Species

Human
Mouse
Rat

Human BID Target Information

Target Symbol: BID, BH3 interacting domain death agonist
Alias Names:
- apoptic death agonist
- BH3 interacting domain death agonist Si6 isoform
- BH3-interacting domain death agonist
- BID isoform ES(1b)
- BID isoform L(2)
- BID isoform Si6
- desmocollin type 4
- FP497
- Human BID coding sequence
- MGC15319
- MGC42355
- p22 BID
NCBI_Gene: NCBI_Gene: 637
UniProtKB: UniProtKB: P55957

Human BID Predicted Functions

Predicted to enable death receptor binding activity. Involved in several processes, including hepatocyte apoptotic process; positive regulation of apoptotic signaling pathway; and positive regulation of cellular component organization. Located in cytosol and mitochondrial outer membrane. Implicated in pancreatic cancer.

Mouse Bid Target Information

Target Symbol: Bid, BH3 interacting domain death agonist
Alias Names:
- 2700049M22Rik
- AI875481
- AU022477
- expressed sequence AI875481
- expressed sequence AU022477
- RIKEN cDNA 2700049M22 gene
NCBI_Gene: NCBI_Gene: 12122
UniProtKB:
- UniprotKB: D3Z463
- UniprotKB: P70444

Mouse Bid Predicted Functions

Enables ubiquitin protein ligase binding activity. Involved in positive regulation of cellular component organization; protein-containing complex assembly; and release of cytochrome c from mitochondria. Acts upstream of or within several processes, including apoptotic signaling pathway; hepatocyte apoptotic process; and regulation of apoptotic process. Located in cytosol; membrane; and mitochondrion. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; hemolymphoid system gland; and liver and biliary system. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human BID (BH3 interacting domain death agonist).

Rat Bid Target Information

Target Symbol: Bid, BH3 interacting domain death agonist
Alias Names:
- apoptotic death agonist BID
- BH3-interacting domain death agonist
- desmocollin type 4
- p22 BID
NCBI_Gene: NCBI_Gene: 64625
UniProtKB:
- UniprotKB: Q9JLT6

Rat Bid Predicted Functions

Predicted to enable ubiquitin protein ligase binding activity. Involved in several processes, including positive regulation of autophagy in response to ER overload; release of cytochrome c from mitochondria; and response to ischemia. Located in cytosol and mitochondrion. Used to study pre-malignant neoplasm. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human BID (BH3 interacting domain death agonist).

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