Human BMP-4 ELISA Kit

$245.00 – $350.00

Related Target	BMP4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.30 pg/mL
Array Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	76% – 103%
Average Recovery	92%

ELISA Kit Detail Information

Related Target	BMP4
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL (diluent)
Sensitivity	0.30 pg/mL
Array Range	3.91 pg/mL – 250 pg/mL
Assay Time	3.5 h
Recovery	76% – 103%
Average Recovery	92%
Intra Precision	1.3% – 3.5%
Inter Precision	4.9% - 8.7%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-BMP-9 monoclonal antibody Human BMP-9 freeze-dried standard Angiotensinogen/AGT/ SerpinA8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human BMP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, BMP-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of BMP-4 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-632 nm).

Related Targets

BMP4

BMP4 Target Infomation Overview

Target Symbol: BMP4, bone morphogenetic protein 4
Gene Groups: Bone morphogenetic proteins
Previous Names: BMP2B

BMP4, bone morphogenetic protein 4 Target Infomation by Species

Human
Mouse
Rat

Human BMP4 Target Information

Target Symbol: BMP4, bone morphogenetic protein 4
Alias:
- BMP-2B
- BMP-4
- BMP2B
- BMP2B1
- bone morphogenetic protein 2B
- bone morphogenetic protein 4 preproprotein
- MCOPS6
- OFC11
- ZYME
NCBI_Gene: 652
UniProtKB: P12644

Human BMP4 Predicted Functions

Enables BMP receptor binding activity; co-receptor binding activity; and receptor ligand activity. Involved in several processes, including animal organ development; negative regulation of cell population proliferation; and transmembrane receptor protein serine/threonine kinase signaling pathway. Acts upstream of or within several processes, including positive regulation of osteoblast differentiation; regulation of transcription by RNA polymerase II; and respiratory system development. Located in extracellular space. Implicated in several diseases, including CAKUT (multiple); cleft lip; orofacial cleft 11; ossification of the posterior longitudinal ligament of spine; and otosclerosis. Biomarker of several diseases, including Barrett’s esophagus; eye disease (multiple); myositis ossificans; progressive osseous heteroplasia; and prolactinoma.

Mouse Bmp4 Target Information

Target Symbol: Bmp4, bone morphogenetic protein 4
Alias:
- Bmp-4
- Bmp2b
- Bmp2b-1
- Bmp2b1
- bone morphogenetic protein 2b
- bone morphogenetic protein 2b 1
NCBI_Gene: 12159
- UniprotKB: P21275
- UniprotKB: Q3ULR1

Mouse Bmp4 Predicted Functions

Enables heparin binding activity. Involved in several processes, including animal organ development; positive regulation of cell population proliferation; and regulation of morphogenesis of a branching structure. Acts upstream of or within several processes, including animal organ development; embryonic morphogenesis; and regulation of animal organ morphogenesis. Located in extracellular space. Is expressed in several structures, including alimentary system; embryo ectoderm; embryo mesenchyme; genitourinary system; and sensory organ. Used to study Axenfeld-Rieger syndrome type 3; atrioventricular septal defect; cleft palate-lateral synechia syndrome; fibrodysplasia ossificans progressiva; and urinary system disease. Human ortholog(s) of this gene implicated in several diseases, including CAKUT (multiple); cleft lip; orofacial cleft 11; ossification of the posterior longitudinal ligament of spine; and otosclerosis. Orthologous to human BMP4 (bone morphogenetic protein 4).

Rat Bmp4 Target Information

Target Symbol: Bmp4, bone morphogenetic protein 4
Alias:
- BMP-2B
- BMP-4
- BOMPR4A
- bone morphogenetic protein 2B
NCBI_Gene: 25296

Rat Bmp4 Predicted Functions

Enables growth factor activity and identical protein binding activity. Involved in several processes, including animal organ development; positive regulation of calcium ion transport; and positive regulation of cell differentiation. Acts upstream of or within positive regulation of ossification and positive regulation of transcription by RNA polymerase II. Located in cytoplasm; extracellular space; and vesicle. Biomarker of Barrett’s esophagus; congenital diaphragmatic hernia; epilepsy; peptic esophagitis; and prolactinoma. Human ortholog(s) of this gene implicated in several diseases, including CAKUT (multiple); cleft lip; orofacial cleft 11; ossification of the posterior longitudinal ligament of spine; and otosclerosis. Orthologous to human BMP4 (bone morphogenetic protein 4).

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