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Human BMP-9 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.19 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 75% – 118% |
| Average Recovery | 1.02 |
| Intra Precision | 3.0% – 4.3% |
| Inter-Precision | 0.9% – 4.4% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-17AF monoclonal antibody Human IL-17AF freeze-dried standard IL-17AF detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IL-17AF antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IL-17AF present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IL-17AF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
GDF2
GDF2 Target Infomation Overview
- Target Symbol: GDF2, growth differentiation factor 2
- Gene Groups: Transforming growth factor beta superfamily
- Alias: BMP-9; BMP9
GDF2, growth differentiation factor 2 Target Infomation by Species
HumanMouseRat
Human GDF2 Target Information
- Target Symbol: GDF2, growth differentiation factor 2
- Alias:
- BMP-9
- BMP9
- bone morphogenetic protein 9
- GDF-2
- growth/differentiation factor 2
- HHT5
- NCBI_Gene: 2658
- UniProtKB: Q9UK05
Human GDF2 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of macromolecule metabolic process; regulation of endothelial cell proliferation; and transmembrane receptor protein serine/threonine kinase signaling pathway. Located in extracellular exosome. Implicated in hereditary hemorrhagic telangiectasia.
Mouse Gdf2 Target Information
- Target Symbol: Gdf2, growth differentiation factor 2
- Alias:
- Bmp9
- bone morphogenetic protein 9
- NCBI_Gene: 12165
Mouse Gdf2 Predicted Functions
Predicted to enable cytokine activity. Involved in several processes, including blood vessel morphogenesis; positive regulation of BMP signaling pathway; and positive regulation of endothelial cell differentiation. Acts upstream of or within osteoblast differentiation; positive regulation of gene expression; and positive regulation of transcription by RNA polymerase II. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including central nervous system; ileum; interphalangeal joint; liver; and male reproductive gland or organ. Human ortholog(s) of this gene implicated in hereditary hemorrhagic telangiectasia. Orthologous to human GDF2 (growth differentiation factor 2).
Rat Gdf2 Target Information
- Target Symbol: Gdf2, growth differentiation factor 2
- Alias:
- growth/differentiation factor 2
- LOC290921
- similar to Growth/differentiation factor 2 precursor (GDF-2) (Bone morphogenetic protein 9) (BMP-9)
- NCBI_Gene: 290921
- UniProtKB: M0RB87
Rat Gdf2 Predicted Functions
Predicted to enable cytokine activity. Predicted to be involved in several processes, including positive regulation of transmembrane receptor protein serine/threonine kinase signaling pathway; regulation of endothelial cell proliferation; and transmembrane receptor protein serine/threonine kinase signaling pathway. Predicted to act upstream of or within osteoblast differentiation; positive regulation of gene expression; and positive regulation of transcription by RNA polymerase II. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in hereditary hemorrhagic telangiectasia. Orthologous to human GDF2 (growth differentiation factor 2).
