Human CCL13/MCP-4 ELISA Kit

$245.00 – $350.00

Related Target	CCL13
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	7.33 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	79% – 119%
Average Recovery	99%

ELISA Kit Detail Information

Related Target	CCL13
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	50 μL
Sensitivity	7.33 pg/mL
Array Range	31.25 pg/mL – 2000 pg/mL
Assay Time	3.5 h
Recovery	79% – 119%
Average Recovery	99%
Intra Precision	3.6% - 5.7%
Inter Precision	4.5% – 11.1%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CCL13/MCP-4 monoclonal antibody Human CCL13/MCP-4 freeze-dried standard CCL13/MCP-4 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CCL13 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CCL13 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CCL13 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CCL13

CCL13 Target Infomation Overview

Target Symbol: CCL13, C-C motif chemokine ligand 13
Gene Groups: Chemokine ligands
Alias: MCP-4; NCC-1; SCYL1; CKb10; MGC17134
Previous Names: SCYA13
Alias Names: small inducible cytokine subfamily A (Cys-Cys), member 13; chemokine (C-C motif) ligand 13

CCL13, C-C motif chemokine ligand 13 Target Infomation by Species

Human
Mouse
Rat

Human CCL13 Target Information

Target Symbol: CCL13, C-C motif chemokine ligand 13
Alias:
- C-C motif chemokine 13
- chemokine (C-C motif) ligand 13
- CK-beta-10
- CKb10
- MCP-4
- MGC17134
- monocyte chemoattractant protein 4
- monocyte chemotactic protein 4
- NCC-1
- NCC1
- new CC chemokine 1
- SCYA13
- SCYL1
- small inducible cytokine subfamily A (Cys-Cys), member 13
- small-inducible cytokine A13
NCBI_Gene: 6357
UniProtKB: Q99616

Human CCL13 Predicted Functions

Enables chemokine activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; eosinophil chemotaxis; and regulation of cell shape. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in multiple sclerosis.

Mouse Ccl11 Target Information

Target Symbol: Ccl11, chemokine (C-C motif) ligand 11
Alias:
- eotaxin
- Scya11
- small inducible cytokine A11
NCBI_Gene: 20292
- UniprotKB: P48298

Mouse Ccl11 Predicted Functions

Predicted to enable CCR3 chemokine receptor binding activity; chemokine activity; and protein dimerization activity. Involved in several processes, including modulation of age-related behavioral decline; negative regulation of neurogenesis; and positive regulation of angiogenesis. Acts upstream of or within branching involved in mammary gland duct morphogenesis and mammary duct terminal end bud growth. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in embryo; endocardial lining; liver parenchyma; liver vasculature; and yolk sac blood vessel. Human ortholog(s) of this gene implicated in asthma; human immunodeficiency virus infectious disease; hyperglycemia; and multiple sclerosis. Orthologous to several human genes including CCL11 (C-C motif chemokine ligand 11).

Rat Ccl12 Target Information

Target Symbol: Ccl12, chemokine (C-C motif) ligand 12
Alias:
- LOC287562
- MCP-5
- monocyte chemotactic protein 5
NCBI_Gene: 287562
UniProtKB: D4ABS1

Rat Ccl12 Predicted Functions

Predicted to enable CCR2 chemokine receptor binding activity and chemokine activity. Involved in negative regulation of leukocyte proliferation and positive regulation of leukocyte migration. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in several diseases, including Henoch-Schoenlein purpura; aggressive periodontitis; autoimmune disease (multiple); gastrointestinal system cancer (multiple); and tuberculosis (multiple). Orthologous to several human genes including CCL8 (C-C motif chemokine ligand 8).

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