Human CNTF ELISA Kit

$350.00$450.00

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TARGETSCNTF
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.13 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery84% – 106%
Average Recovery0.96
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity4.13 pg/mL
Range31.25 pg/mL – 2000 pg/mL
Assay Time3.5 h
Recovery84% – 106%
Average Recovery0.96
Intra Precision4.1% – 8.0%
Inter-Precision2.2% – 5.5%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CNTF monoclonal antibody
Human CNTF freeze-dried standard
CNTF detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CNTF antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CNTF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CNTF in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CNTF

CNTF Target Infomation Overview

  • Target Symbol: CNTF, ciliary neurotrophic factor
  • Gene Groups: Interleukin 6 type cytokine family
  • Alias: HCNTF
  • Alias Names: Ciliary Neuronotrophic Factor

CNTF, ciliary neurotrophic factor Target Infomation by Species

HumanMouseRat

Human CNTF Target Information

Human CNTF Predicted Functions

Enables growth factor activity and interleukin-6 receptor binding activity. Involved in ciliary neurotrophic factor-mediated signaling pathway; negative regulation of neuron apoptotic process; and positive regulation of tyrosine phosphorylation of STAT protein. Acts upstream of or within positive regulation of gene expression. Predicted to be located in extracellular region. Predicted to be active in axon and extracellular space. Implicated in Huntington’s disease; disease of mental health; glaucoma; multiple sclerosis; and obesity. Biomarker of keratoconus.

Mouse Cntf Target Information

Mouse Cntf Predicted Functions

Predicted to enable signaling receptor binding activity. Acts upstream of or within several processes, including negative regulation of photoreceptor cell differentiation; positive regulation of tyrosine phosphorylation of STAT protein; and regulation of retinal cell programmed cell death. Located in extracellular space. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Huntington’s disease; disease of mental health; glaucoma; multiple sclerosis; and obesity. Orthologous to human CNTF (ciliary neurotrophic factor).

Rat Cntf Target Information

Rat Cntf Predicted Functions

Enables ciliary neurotrophic factor receptor binding activity and cytokine activity. Involved in several processes, including membrane hyperpolarization; neurogenesis; and positive regulation of cell communication. Located in several cellular components, including axon; neuronal cell body; and plasma membrane bounded cell projection cytoplasm. Used to study Huntington’s disease and glaucoma. Biomarker of hyperglycemia and retinitis pigmentosa. Human ortholog(s) of this gene implicated in Huntington’s disease; disease of mental health; glaucoma; multiple sclerosis; and obesity. Orthologous to human CNTF (ciliary neurotrophic factor).

CNTF Target News

    Multiple Sclerosis

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