Human Coagulation Factor IX/ FIX/F9 ELISA Kit

$350.00$450.00

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TARGETSF9
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity53.50 pg/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery78% – 122%
Average Recovery1.05
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity53.50 pg/mL
Range0.63 ng/mL – 40 ng/mL
Assay Time3.5 h
Recovery78% – 122%
Average Recovery1.05
Intra Precision2.6% – 4.3%
Inter-Precision2.0% – 6.7%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Coagulation Factor IX/ FIX/F9 monoclonal antibody
Human Coagulation Factor IX/ FIX/F9 freeze-dried standard
Coagulation Factor IX/ FIX/F9 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human F9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, F9 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of F9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

F9

F9 Target Infomation Overview

  • Target Symbol: F9, coagulation factor IX
  • Gene Groups: Gla domain containing
  • Alias: FIX
  • Alias Names: Factor IX; plasma thromboplastic component; Christmas disease; hemophilia B

F9, coagulation factor IX Target Infomation by Species

HumanMouseRat

Human F9 Target Information

  • Target Symbol: F9, coagulation factor IX
  • Alias:
    • Christmas factor
    • coagulation factor 9
    • coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
    • F9 p22
    • factor 9
    • factor IX F9
    • FIX
    • FIX F9
    • HEMB
    • MGC129641
    • MGC129642
    • P19
    • plasma thromboplastic component
    • plasma thromboplastin component
    • PTC
    • serine protease
    • THPH8
  • NCBI_Gene: 2158
  • UniProtKB: P00740

Human F9 Predicted Functions

Enables calcium ion binding activity and endopeptidase activity. Involved in blood coagulation and zymogen activation. Located in extracellular space. Part of collagen-containing extracellular matrix. Implicated in X-linked thrombophilia due to factor IX defect; X-linked warfarin sensitivity; and hemophilia B. Biomarker of type 2 diabetes mellitus.

Mouse F9 Target Information

Mouse F9 Predicted Functions

Enables peptidase activity. Acts upstream of or within blood coagulation. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in embryo and liver. Used to study hemophilia B. Human ortholog(s) of this gene implicated in X-linked thrombophilia due to factor IX defect; X-linked warfarin sensitivity; and hemophilia B. Orthologous to human F9 (coagulation factor IX).

Rat F9 Target Information

  • Target Symbol: F9, coagulation factor IX
  • Alias:
    • christmas factor
    • coagulation factor 9
    • Coagulation factor IX (plasma thromboplastic component Christmas disease hemophilia B)
    • Coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
  • NCBI_Gene: 24946
  • UniProtKB: P16296

Rat F9 Predicted Functions

Predicted to enable calcium ion binding activity and endopeptidase activity. Involved in blood coagulation. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in X-linked thrombophilia due to factor IX defect; X-linked warfarin sensitivity; and hemophilia B. Orthologous to human F9 (coagulation factor IX).

F9 Target News

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