Product Category
Newest Products
Human Coagulation Factor IX/ FIX/F9 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 53.50 pg/mL |
| Range | 0.63 ng/mL – 40 ng/mL |
| Assay Time | 3.5 h |
| Recovery | 78% – 122% |
| Average Recovery | 1.05 |
| Intra Precision | 2.6% – 4.3% |
| Inter-Precision | 2.0% – 6.7% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Coagulation Factor IX/ FIX/F9 monoclonal antibody Human Coagulation Factor IX/ FIX/F9 freeze-dried standard Coagulation Factor IX/ FIX/F9 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human F9 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, F9 present in the samples binds to the solid-phase antibody and the detection antibody. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of F9 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
F9
F9 Target Infomation Overview
- Target Symbol: F9, coagulation factor IX
- Gene Groups: Gla domain containing
- Alias: FIX
- Alias Names: Factor IX; plasma thromboplastic component; Christmas disease; hemophilia B
F9, coagulation factor IX Target Infomation by Species
HumanMouseRat
Human F9 Target Information
- Target Symbol: F9, coagulation factor IX
- Alias:
- Christmas factor
- coagulation factor 9
- coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
- F9 p22
- factor 9
- factor IX F9
- FIX
- FIX F9
- HEMB
- MGC129641
- MGC129642
- P19
- plasma thromboplastic component
- plasma thromboplastin component
- PTC
- serine protease
- THPH8
- NCBI_Gene: 2158
- UniProtKB: P00740
Human F9 Predicted Functions
Enables calcium ion binding activity and endopeptidase activity. Involved in blood coagulation and zymogen activation. Located in extracellular space. Part of collagen-containing extracellular matrix. Implicated in X-linked thrombophilia due to factor IX defect; X-linked warfarin sensitivity; and hemophilia B. Biomarker of type 2 diabetes mellitus.
Mouse F9 Target Information
- Target Symbol: F9, coagulation factor IX
- Alias:
- AW111646
- Cf-9
- Cf9
- expressed sequence AW111646
- NCBI_Gene: 14071
Mouse F9 Predicted Functions
Enables peptidase activity. Acts upstream of or within blood coagulation. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in embryo and liver. Used to study hemophilia B. Human ortholog(s) of this gene implicated in X-linked thrombophilia due to factor IX defect; X-linked warfarin sensitivity; and hemophilia B. Orthologous to human F9 (coagulation factor IX).
Rat F9 Target Information
- Target Symbol: F9, coagulation factor IX
- Alias:
- christmas factor
- coagulation factor 9
- Coagulation factor IX (plasma thromboplastic component Christmas disease hemophilia B)
- Coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B)
- NCBI_Gene: 24946
- UniProtKB: P16296
Rat F9 Predicted Functions
Predicted to enable calcium ion binding activity and endopeptidase activity. Involved in blood coagulation. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in X-linked thrombophilia due to factor IX defect; X-linked warfarin sensitivity; and hemophilia B. Orthologous to human F9 (coagulation factor IX).
