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Human Complement Factor D/ Adipsin ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 10 μL |
| Sensitivity | 0.11 pg/mL |
| Range | 7.81 pg/mL – 500 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 118% |
| Average Recovery | 1.07 |
| Intra Precision | 3.9% – 4.5% |
| Inter-Precision | 3.7% – 4.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Complement Factor D/ Adipsin monoclonal antibody Human Complement Factor D/ Adipsin freeze-dried standard Complement Factor D/ Adipsin detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CFD antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, CFD present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CFD in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
CFD
CFD Target Infomation Overview
- Target Symbol: CFD, complement factor D
- Gene Groups: Granule associated serine proteases of immune defence; Complement system activation components
- Alias: ADN
- Previous Names: DF; PFD
- Alias Names: adipsin; D component of complement (adipsin); properdin factor D; complement factor D (adipsin)
CFD, complement factor D Target Infomation by Species
HumanMouseRat
Human CFD Target Information
- Target Symbol: CFD, complement factor D
- Alias:
- ADIPSIN
- ADN
- C3 convertase activator
- complement factor D (adipsin)
- complement factor D preproprotein
- D component of complement (adipsin)
- DF
- PFD
- properdin factor D
- NCBI_Gene: 1675
- UniProtKB: P00746
Human CFD Predicted Functions
Predicted to enable serine-type endopeptidase activity. Predicted to be involved in complement activation. Predicted to act upstream of or within several processes, including complement activation, alternative pathway; retina development in camera-type eye; and vascular associated smooth muscle cell differentiation. Located in extracellular exosome. Biomarker of obesity.
Mouse Cfd Target Information
- Target Symbol: Cfd, complement factor D (adipsin)
- Alias:
- adipsin
- Adn
- D component (adipsin) of complement
- DF
- factor D
- NCBI_Gene: 11537
Mouse Cfd Predicted Functions
Predicted to enable endopeptidase activity. Acts upstream of or within several processes, including complement activation, alternative pathway; retina development in camera-type eye; and vascular associated smooth muscle cell differentiation. Located in extracellular space. Is expressed in adipose tissue; brown fat; epididymal fat pad; and white fat. Orthologous to human CFD (complement factor D).
Rat Cfd Target Information
- Target Symbol: Cfd, complement factor D
- Alias:
- adipsin
- Adn
- C3 convertase activator
- complement factor D (adipsin)
- D component of complement (adipsin)
- Df
- endogenous vascular elastase
- EVE
- properdin factor D
- NCBI_Gene: 54249
Rat Cfd Predicted Functions
Enables endopeptidase activity. Involved in Notch signaling pathway. Located in extracellular space. Used to study arthus reaction. Biomarker of obesity. Orthologous to human CFD (complement factor D).
