Human CXCL-10/IP-10 ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL |
| Sensitivity | 3.50 pg/mL |
| Array Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 78% – 114% |
| Average Recovery | 93% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | human |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 50 μL |
| Sensitivity | 3.50 pg/mL |
| Array Range | 31.25 pg/mL – 2000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 78% – 114% |
| Average Recovery | 93% |
| Intra Precision | 3.0% – 5.0% |
| Inter Precision | 3.8% – 6.1% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL10/IP-10 monoclonal antibody Human CXCL10/IP-10 freeze-dried standard CXCL10/IP-10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IP-10 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IP-10 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IP-10 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
CXCL10
CXCL10 Target Infomation Overview
- Target Symbol: CXCL10, C-X-C motif chemokine ligand 10
- Gene Groups: Chemokine ligands
- Alias: IFI10; IP-10; crg-2; mob-1; C7; gIP-10
- Previous Names: INP10; SCYB10
- Alias Names: small inducible cytokine subfamily B (Cys-X-Cys), member 10; chemokine (C-X-C motif) ligand 10
CXCL10, C-X-C motif chemokine ligand 10 Target Infomation by Species
- Human
- Mouse
- Rat
Human CXCL10 Target Information
- Target Symbol: CXCL10, C-X-C motif chemokine ligand 10
- Alias:
- 10 kDa interferon gamma-induced protein
- C-X-C motif chemokine 10
- C7
- chemokine (C-X-C motif) ligand 10
- crg-2
- gamma IP10
- gamma-IP10
- gIP-10
- IFI10
- INP10
- interferon-inducible cytokine IP-10
- IP-10
- mob-1
- protein 10 from interferon (gamma)-induced cell line
- SCYB10
- small inducible cytokine subfamily B (Cys-X-Cys), member 10
- small-inducible cytokine B10
- NCBI_Gene: 3627
- UniProtKB: P02778
Human CXCL10 Predicted Functions
Enables chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Involved in several processes, including positive regulation of release of sequestered calcium ion into cytosol; regulation of leukocyte chemotaxis; and response to virus. Acts upstream of or within cellular response to lipopolysaccharide and positive regulation of T cell migration. Located in extracellular region. Implicated in hepatitis B and middle cerebral artery infarction. Biomarker of several diseases, including artery disease (multiple); diabetes mellitus (multiple); fatty liver disease (multiple); liver disease (multiple); and lung disease (multiple).
Mouse Cxcl10 Target Information
- Target Symbol: Cxcl10, chemokine (C-X-C motif) ligand 10
- Alias:
- C7
- CRG-2
- gIP-10
- Ifi10
- INP10
- interferon activated gene 10
- IP-10
- IP10
- mob-1
- Scyb10
- small inducible cytokine B subfamily (Cys-X-Cys), member 10
- NCBI_Gene: 15945
Mouse Cxcl10 Predicted Functions
Predicted to enable chemoattractant activity; chemokine receptor binding activity; and heparin binding activity. Acts upstream of or within several processes, including defense response to virus; negative regulation of myoblast differentiation; and negative regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including alimentary system; axial skeleton; hemolymphoid system; and liver. Human ortholog(s) of this gene implicated in hepatitis B; middle cerebral artery infarction; and type 1 diabetes mellitus. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).
Rat Cxcl10 Target Information
- Target Symbol: Cxcl10, C-X-C motif chemokine ligand 10
- Alias:
- 10 kDa interferon gamma-induced protein
- C-X-C motif chemokine 10
- chemokine (C-X-C motif) ligand 10
- gamma-IP10
- interferon-inducible cytokine IP-10
- interferon-inducible protein 10
- IP-10
- protein Mob-1
- Scyb10
- small inducible cytokine B subfamily (Cys-X-Cys) member 10
- small inducible cytokine B subfamily (Cys-X-Cys), member 10
- small-inducible cytokine B10
- NCBI_Gene: 245920
- UniProtKB: P48973
Rat Cxcl10 Predicted Functions
Enables chemokine activity. Involved in several processes, including response to auditory stimulus; response to gamma radiation; and response to vitamin D. Located in extracellular space. Used to study adult respiratory distress syndrome; myocarditis; proteinuria; renovascular hypertension; and type 1 diabetes mellitus. Biomarker of brain ischemia; encephalitis; and uveitis. Human ortholog(s) of this gene implicated in hepatitis B and middle cerebral artery infarction. Orthologous to human CXCL10 (C-X-C motif chemokine ligand 10).
Products with the Same Target - CXCL10
Citations for Human CXCL-10/IP-10 ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK8100
- ELISA Kit
Cortisol Competitive ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1122
- ELISA Kit
Human Angiopoietin-1/ANG-1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1215
- ELISA Kit
Human Angiopoietin-2/ANG-2 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1317
- ELISA Kit
Human ACE ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1260
- ELISA Kit
Human alpha-Galactosidase A/GLA ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK195
- ELISA Kit
Human Adiponectin ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.