Human CXCL1/KC ELISA Kit

$350.00$450.00

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TARGETSCXCL1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity0.10 pg/mL
Range0.78 pg/mL – 50 pg/mL
Assay Time3.5 h
Recovery73% – 102%
Average Recovery0.92
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL(diluent)
Sensitivity0.10 pg/mL
Range0.78 pg/mL – 50 pg/mL
Assay Time3.5 h
Recovery73% – 102%
Average Recovery0.92
Intra Precision4.7% – 8.4%
Inter-Precision2.8% – 11.0%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL1/CK monoclonal antibody
Human CXCL1/CK freeze-dried standard
Angiotensinogen/AGT/ SerpinA8 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CXCL1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

CXCL1

CXCL1 Target Infomation Overview

  • Target Symbol: CXCL1, C-X-C motif chemokine ligand 1
  • Gene Groups: Chemokine ligands
  • Alias: SCYB1; GROa; MGSA-a; NAP-3
  • Previous Names: MGSA; GRO1; FSP
  • Alias Names: melanoma growth stimulating activity, alpha; GRO1 oncogene (melanoma growth stimulating activity, alpha); fibroblast secretory protein; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

CXCL1, C-X-C motif chemokine ligand 1 Target Infomation by Species

HumanMouseRat

Human CXCL1 Target Information

  • Target Symbol: CXCL1, C-X-C motif chemokine ligand 1
  • Alias:
    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • fibroblast secretory protein
    • FSP
    • GRO-alpha(1-73)
    • GRO1
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • growth-regulated alpha protein
    • melanoma growth stimulating activity, alpha
    • melanoma growth stimulatory activity alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • NAP-3
    • neutrophil-activating protein 3
    • SCYB1
  • NCBI_Gene: 2919
  • UniProtKB: P09341

Human CXCL1 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Predicted to be located in extracellular region; specific granule lumen; and tertiary granule lumen. Predicted to be active in extracellular space. Biomarker of asthma; chronic obstructive pulmonary disease; common cold; cystic fibrosis; and hyperhomocysteinemia.

Mouse Cxcl1 Target Information

Mouse Cxcl1 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in positive regulation of hematopoietic stem cell proliferation; positive regulation of neutrophil mediated killing of fungus; and positive regulation of superoxide anion generation. Located in extracellular space. Is expressed in several structures, including gonad; liver; lung; nervous system; and retina. Orthologous to human CXCL2 (C-X-C motif chemokine ligand 2).

Rat Cxcl1 Target Information

  • Target Symbol: Cxcl1, C-X-C motif chemokine ligand 1
  • Alias:
    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1
    • chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • CINC-1
    • cytokine-induced neutrophil chemoattractant 1
    • Gro
    • Gro1
    • growth-regulated alpha protein
    • platelet-derived growth factor-inducible protein KC
  • NCBI_Gene: 81503

Rat Cxcl1 Predicted Functions

Enables chemokine activity. Involved in several processes, including neutrophil chemotaxis; positive regulation of ion transport; and response to amphetamine. Located in extracellular space. Biomarker of several diseases, including acute necrotizing pancreatitis; pneumonia (multiple); prostatitis; severe acute respiratory syndrome; and status epilepticus. Orthologous to human CXCL1 (C-X-C motif chemokine ligand 1); CXCL2 (C-X-C motif chemokine ligand 2); and CXCL3 (C-X-C motif chemokine ligand 3).

CXCL1 Target News

    2.1
    The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A
    Open Medicine
    28.2
    An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma
    Cancer Discovery
    8.185
    CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway
    Journal of Molecular Cell Biology
    Cardiovascular Disease (Hypertension & Atherosclerosis & Acute Myocardial Infarction & Coronary Heart Disease)
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis

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