Human CXCL1/KC ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	CXCL1
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	0.10 pg/mL
Range	0.78 pg/mL – 50 pg/mL
Assay Time	3.5 h
Recovery	73% – 102%
Average Recovery	0.92

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL(diluent)
Sensitivity	0.10 pg/mL
Range	0.78 pg/mL – 50 pg/mL
Assay Time	3.5 h
Recovery	73% – 102%
Average Recovery	0.92
Intra Precision	4.7% – 8.4%
Inter-Precision	2.8% – 11.0%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL1/CK monoclonal antibody Human CXCL1/CK freeze-dried standard Angiotensinogen/AGT/ SerpinA8 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL1 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CXCL1 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL1 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

CXCL1

CXCL1 Target Infomation Overview

Target Symbol: CXCL1, C-X-C motif chemokine ligand 1
Gene Groups: Chemokine ligands
Alias: SCYB1; GROa; MGSA-a; NAP-3
Previous Names: MGSA; GRO1; FSP
Alias Names: melanoma growth stimulating activity, alpha; GRO1 oncogene (melanoma growth stimulating activity, alpha); fibroblast secretory protein; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

CXCL1, C-X-C motif chemokine ligand 1 Target Infomation by Species

HumanMouseRat

Human CXCL1 Target Information

Target Symbol: CXCL1, C-X-C motif chemokine ligand 1
Alias:
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
- fibroblast secretory protein
- FSP
- GRO-alpha(1-73)
- GRO1
- GRO1 oncogene (melanoma growth stimulating activity, alpha)
- GRO1 oncogene (melanoma growth-stimulating activity)
- GROa
- growth-regulated alpha protein
- melanoma growth stimulating activity, alpha
- melanoma growth stimulatory activity alpha
- MGSA
- MGSA alpha
- MGSA-a
- NAP-3
- neutrophil-activating protein 3
- SCYB1
NCBI_Gene: 2919
UniProtKB: P09341

Human CXCL1 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Predicted to be located in extracellular region; specific granule lumen; and tertiary granule lumen. Predicted to be active in extracellular space. Biomarker of asthma; chronic obstructive pulmonary disease; common cold; cystic fibrosis; and hyperhomocysteinemia.

Mouse Cxcl1 Target Information

Target Symbol: Cxcl1, chemokine (C-X-C motif) ligand 1
Alias:
- fibroblast secretory protein
- Fsp
- Gro1
- GRO1 oncogene
- KC
- KC/GRO-alpha
- melanoma growth stimulatory activity
- Mgsa
- N51
- Scyb1
NCBI_Gene: 14825

Mouse Cxcl1 Predicted Functions

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in positive regulation of hematopoietic stem cell proliferation; positive regulation of neutrophil mediated killing of fungus; and positive regulation of superoxide anion generation. Located in extracellular space. Is expressed in several structures, including gonad; liver; lung; nervous system; and retina. Orthologous to human CXCL2 (C-X-C motif chemokine ligand 2).

Rat Cxcl1 Target Information

Target Symbol: Cxcl1, C-X-C motif chemokine ligand 1
Alias:
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
- CINC-1
- cytokine-induced neutrophil chemoattractant 1
- Gro
- Gro1
- growth-regulated alpha protein
- platelet-derived growth factor-inducible protein KC
NCBI_Gene: 81503
- UniprotKB: G3V6C6
- UniprotKB: P14095

Rat Cxcl1 Predicted Functions

Enables chemokine activity. Involved in several processes, including neutrophil chemotaxis; positive regulation of ion transport; and response to amphetamine. Located in extracellular space. Biomarker of several diseases, including acute necrotizing pancreatitis; pneumonia (multiple); prostatitis; severe acute respiratory syndrome; and status epilepticus. Orthologous to human CXCL1 (C-X-C motif chemokine ligand 1); CXCL2 (C-X-C motif chemokine ligand 2); and CXCL3 (C-X-C motif chemokine ligand 3).