EK1254

Human CXCL16 ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity1.09 pg/mL
Array Range3.13 pg/mL – 200 pg/mL
Assay Time3.5 h
Recovery81% – 105%
Average Recovery98%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume100 μL
Sensitivity1.09 pg/mL
Array Range3.13 pg/mL – 200 pg/mL
Assay Time3.5 h
Recovery81% – 105%
Average Recovery98%
Intra Precision4.3% – 5.7%
Inter Precision5.5% – 7.3%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-CXCL16 monoclonal antibody
Human CXCL16 freeze-dried standard
CXCL16 detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human CXCL16 antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the CXCL16 present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of CXCL16 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

CXCL16

CXCL16 Target Infomation Overview

  • Target Symbol: CXCL16, C-X-C motif chemokine ligand 16
  • Gene Groups: Chemokine ligands; Scavenger receptors
  • Alias: SR-PSOX; CXCLG16; SRPSOX
  • Alias Names: CXC chemokine ligand 16; chemokine (C-X-C motif) ligand 16

CXCL16, C-X-C motif chemokine ligand 16 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human CXCL16 Target Information

  • Target Symbol: CXCL16, C-X-C motif chemokine ligand 16
  • Alias:
    • C-X-C motif chemokine 16
    • chemokine (C-X-C motif) ligand 16
    • CXC chemokine ligand 16
    • CXCLG16
    • scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
    • small-inducible cytokine B16
    • SR-PSOX
    • SRPSOX
    • transmembrane chemokine CXCL16
  • NCBI_Gene: 58191
  • UniProtKB: Q9H2A7

Human CXCL16 Predicted Functions

Enables chemokine activity. Involved in several processes, including positive regulation of cell growth; response to interferon-gamma; and response to tumor necrosis factor. Located in extracellular space. Biomarker of COVID-19 and systemic scleroderma.

Mouse Cxcl16 Target Information

Mouse Cxcl16 Predicted Functions

Enables chemokine activity; low-density lipoprotein particle receptor activity; and scavenger receptor activity. Involved in T cell chemotaxis and cellular response to lipopolysaccharide. Located in extracellular space. Is integral component of membrane. Is expressed in bladder; female associated reproductive structure; hindbrain meninges; male associated reproductive structure; and spinal cord meninges. Orthologous to human CXCL16 (C-X-C motif chemokine ligand 16).

Rat Cxcl16 Target Information

Rat Cxcl16 Predicted Functions

Predicted to enable chemokine activity; low-density lipoprotein particle receptor activity; and scavenger receptor activity. Predicted to be involved in several processes, including T cell chemotaxis; cellular response to lipopolysaccharide; and response to interferon-gamma. Predicted to be integral component of membrane. Predicted to be active in extracellular space. Orthologous to human CXCL16 (C-X-C motif chemokine ligand 16).

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