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Human Dkk-1 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 2.97 pg/mL |
| Range | 62.50 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 78% – 128% |
| Average Recovery | 1 |
| Intra Precision | 3.9% – 4.7% |
| Inter-Precision | 4.5% – 6.6% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Dkk-1 monoclonal antibody Human Dkk-1 freeze-dried standard Dkk-1 detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Dkk-1 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Dkk-1 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, the TMB chromogenic substrate is added, and color development is carried out while avoiding light. The intensity of the color reaction is directly proportional to the concentration of Dkk-1 in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
DKK1
DKK1 Target Infomation Overview
- Target Symbol: DKK1, dickkopf WNT signaling pathway inhibitor 1
- Alias: SK; DKK-1
- Alias Names: dickkopf (Xenopus laevis) homolog 1; dickkopf 1 homolog (Xenopus laevis)
DKK1, dickkopf WNT signaling pathway inhibitor 1 Target Infomation by Species
HumanMouseRat
Human DKK1 Target Information
- Target Symbol: DKK1, dickkopf WNT signaling pathway inhibitor 1
- Alias:
- dickkopf 1 homolog
- dickkopf related protein-1
- dickkopf-1 like
- dickkopf-like protein 1
- dickkopf-related protein 1
- DKK-1
- hDkk-1
- SK
- NCBI_Gene: 22943
- UniProtKB: O94907
Human DKK1 Predicted Functions
Enables co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in several processes, including regulation of cell fate specification; regulation of gene expression; and regulation of protein phosphorylation. Located in extracellular space and plasma membrane. Implicated in anodontia.
Mouse Dkk1 Target Information
- Target Symbol: Dkk1, dickkopf WNT signaling pathway inhibitor 1
- Alias:
- mdkk-1
- NCBI_Gene: 13380
Mouse Dkk1 Predicted Functions
Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in several processes, including modulation of age-related behavioral decline; negative regulation of cellular component organization; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including Wnt signaling pathway involved in somitogenesis; embryonic morphogenesis; and negative regulation of signal transduction. Predicted to be located in extracellular region and plasma membrane. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; embryo mesenchyme; eye; genitourinary system; and nervous system. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).
Rat Dkk1 Target Information
- Target Symbol: Dkk1, dickkopf WNT signaling pathway inhibitor 1
- Alias:
- dickkopf 1 homolog
- dickkopf 1 homolog (Xenopus laevis)
- dickkopf homolog 1
- dickkopf homolog 1 (Xenopus laevis)
- dickkopf-like protein 1
- dickkopf-related protein 1
- LOC293897
- NEWGENE_1307313
- NCBI_Gene: 293897
- UniProtKB: D3Z9J1
Rat Dkk1 Predicted Functions
Predicted to enable co-receptor binding activity; low-density lipoprotein particle receptor binding activity; and receptor antagonist activity. Involved in negative regulation of ossification and positive regulation of neuron death. Located in extracellular space. Used to study visual epilepsy. Human ortholog(s) of this gene implicated in anodontia. Orthologous to human DKK1 (dickkopf WNT signaling pathway inhibitor 1).
