Human EGFR/HER1/ErbB1 ELISA Kit

ELISA Kits

$350.00 – $450.00

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Datasheet

SDS File

TARGETS	EGFR
SPECIES	Human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	5.79 pg/mL
Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	87% – 119%
Average Recovery	1.06

Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	5.79 pg/mL
Range	78.13 pg/mL – 5000 pg/mL
Assay Time	3.5 h
Recovery	87% – 119%
Average Recovery	1.06
Intra Precision	2.2% – 3.5%
Inter-Precision	2.3% – 4.4%
Platform	ELISA
Plate	Detachable 96-well plate
Size	96T/48T
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-EGFR/HER1/ErbB1 monoclonal antibody Human EGFR/HER1/ErbB1 freeze-dried standard EGFR/HER1/ErbB1 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human EGFR antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, EGFR present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of EGFR in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Targets

EGFR

EGFR Target Infomation Overview

Target Symbol: EGFR, epidermal growth factor receptor
Gene Groups: Erb-b2 receptor tyrosine kinases
Alias: ERBB1; ERRP
Previous Names: ERBB
Alias Names: erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian); erb-b2 receptor tyrosine kinase 1; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)

EGFR, epidermal growth factor receptor Target Infomation by Species

HumanMouseRat

Human EGFR Target Information

Target Symbol: EGFR, epidermal growth factor receptor
Alias:
- avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
- cell growth inhibiting protein 40
- cell proliferation-inducing protein 61
- EGFR vIII
- epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
- epidermal growth factor receptor tyrosine kinase domain
- erb-b2 receptor tyrosine kinase 1
- ERBB
- ERBB1
- ERRP
- erythroblastic leukemia viral (v-erb-b) oncogene homolog
- HER1
- mENA
- NISBD2
- PIG61
- proto-oncogene c-ErbB-1
- receptor tyrosine-protein kinase erbB-1
NCBI_Gene: 1956
UniProtKB: P00533

Human EGFR Predicted Functions

Enables several functions, including actin filament binding activity; enzyme binding activity; and epidermal growth factor-activated receptor activity. Contributes to nitric-oxide synthase regulator activity. Involved in several processes, including positive regulation of macromolecule metabolic process; regulation of intracellular signal transduction; and regulation of protein metabolic process. Acts upstream of or within epidermal growth factor receptor signaling pathway and positive regulation of cell population proliferation. Located in several cellular components, including basolateral plasma membrane; endosome; and membrane raft. Part of receptor complex. Is active in ruffle membrane. Colocalizes with endosome. Implicated in several diseases, including colorectal cancer; lung non-small cell carcinoma (multiple); pancreatic cancer; prostate cancer; and pulmonary tuberculosis. Biomarker of several diseases, including Human papillomavirus infectious disease; breast cancer (multiple); carcinoma (multiple); chronic obstructive pulmonary disease; and pulmonary hypertension.

Mouse Egfr Target Information

Target Symbol: Egfr, epidermal growth factor receptor
Alias:
- 9030024J15Rik
- AI552599
- avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
- avian erythroblastosis oncogene B
- Erbb
- Errb1
- expressed sequence AI552599
- RIKEN cDNA 9030024J15 gene
- wa-2
- wa2
- Wa5
- waved 2
- waved 5
NCBI_Gene: 13649

Mouse Egfr Predicted Functions

Enables epidermal growth factor binding activity and epidermal growth factor-activated receptor activity. Involved in cellular response to amino acid stimulus and positive regulation of fibroblast proliferation. Acts upstream of or within several processes, including animal organ development; epidermal growth factor receptor signaling pathway; and protein autophosphorylation. Located in several cellular components, including basolateral plasma membrane; endocytic vesicle; and perinuclear region of cytoplasm. Is expressed in several structures, including alimentary system; brain; integumental system; limb; and respiratory system. Used to study Coronavirus infectious disease and aortic valve disease. Human ortholog(s) of this gene implicated in several diseases, including colorectal cancer; lung non-small cell carcinoma (multiple); pancreatic cancer; prostate cancer; and pulmonary tuberculosis. Orthologous to human EGFR (epidermal growth factor receptor).

Rat Egfr Target Information

Target Symbol: Egfr, epidermal growth factor receptor
Alias:
- avian erythroblastic leukemia viral (v-erbB) oncogene homolog
- Egfr-related peptide
- Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1)
- Epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1)
- ErbB-1
- ERBB1
- Errp
NCBI_Gene: 24329

Rat Egfr Predicted Functions

Enables several functions, including calmodulin binding activity; epidermal growth factor binding activity; and epidermal growth factor-activated receptor activity. Involved in several processes, including animal organ development; neurogenesis; and positive regulation of cell communication. Located in several cellular components, including apical plasma membrane; basolateral plasma membrane; and cytoplasmic vesicle. Used to study bronchiolo-alveolar adenocarcinoma; dilated cardiomyopathy; and high grade glioma. Biomarker of several diseases, including Parkinsonism; acute kidney failure; lung disease (multiple); median neuropathy; and peptic esophagitis. Human ortholog(s) of this gene implicated in several diseases, including colorectal cancer; lung non-small cell carcinoma (multiple); pancreatic cancer; prostate cancer; and pulmonary tuberculosis. Orthologous to human EGFR (epidermal growth factor receptor).