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Human Endoglin/CD105 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 100 μL |
| Sensitivity | 3.49 pg/mL |
| Range | 62.5 pg/mL – 4000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 95% – 119% |
| Average Recovery | 1.08 |
| Intra Precision | 2.7% – 4.4% |
| Inter-Precision | 3.0% – 3.5% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Endoglin/CD105 monoclonal antibody Human Endoglin/CD105 freeze-dried standard Endoglin/CD105 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Endoglin antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Endoglin present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Endoglin in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
ENG
ENG Target Infomation Overview
- Target Symbol: ENG, endoglin
- Gene Groups: CD molecules
- Alias: END; HHT1; CD105
- Previous Names: ORW1; ORW
- Alias Names: Osler-Rendu-Weber syndrome 1
ENG, endoglin Target Infomation by Species
HumanMouseRat
Human ENG Target Information
- Target Symbol: ENG, endoglin
- Alias:
- CD105
- CD105 antigen
- END
- FLJ41744
- HHT1
- ORW
- ORW1
- NCBI_Gene: 2022
- UniProtKB: P17813
Human ENG Predicted Functions
Enables several functions, including galactose binding activity; protein homodimerization activity; and transforming growth factor beta receptor binding activity. Contributes to BMP binding activity and transforming growth factor beta binding activity. Involved in several processes, including central nervous system vasculogenesis; regulation of transcription by RNA polymerase II; and regulation of transmembrane receptor protein serine/threonine kinase signaling pathway. Located in external side of plasma membrane; extracellular space; and nuclear body. Part of receptor complex. Implicated in arteriovenous malformation; arteriovenous malformations of the brain; breast cancer; hereditary hemorrhagic telangiectasia; and intracranial aneurysm. Biomarker of several diseases, including artery disease (multiple); clear cell renal cell carcinoma; diabetic retinopathy; hematologic cancer (multiple); and secondary hyperparathyroidism.
Mouse Eng Target Information
- Target Symbol: Eng, endoglin
- Alias:
- AI528660
- AI662476
- CD105
- Endo
- expressed sequence AI528660
- expressed sequence AI662476
- NCBI_Gene: 13805
Mouse Eng Predicted Functions
Enables protein homodimerization activity and transforming growth factor beta binding activity. Involved in several processes, including circulatory system development; positive regulation of cell differentiation; and regulation of gene expression. Acts upstream of or within circulatory system development and positive regulation of angiogenesis. Located in endothelial microparticle. Is expressed in several structures, including brain; cardiovascular system; extraembryonic vascular system; and lung. Used to study arteriovenous malformations of the brain and hereditary hemorrhagic telangiectasia. Human ortholog(s) of this gene implicated in arteriovenous malformation; arteriovenous malformations of the brain; breast cancer; hereditary hemorrhagic telangiectasia; and intracranial aneurysm. Orthologous to human ENG (endoglin).
Rat Eng Target Information
- Target Symbol: Eng, endoglin
- Alias:
- MGC124909
- NCBI_Gene: 497010
Rat Eng Predicted Functions
Enables type II transforming growth factor beta receptor binding activity. Involved in several processes, including cellular response to mechanical stimulus; positive regulation of collagen biosynthetic process; and positive regulation of macromolecule metabolic process. Located in cell surface and extracellular space. Used to study congestive heart failure; pre-eclampsia; and renal fibrosis. Biomarker of cholestasis; congestive heart failure; hepatocellular carcinoma; hypertension (multiple); and renal fibrosis. Human ortholog(s) of this gene implicated in arteriovenous malformation; arteriovenous malformations of the brain; breast cancer; hereditary hemorrhagic telangiectasia; and intracranial aneurysm. Orthologous to human ENG (endoglin).
