Human Fetuin-A ELISA?Kit

$245.00 – $350.00

Related Target	AHSG
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	2.22 pg/mL
Array Range	46.88 pg/mL – 3000 pg/mL
Assay Time	3.5 h
Recovery	88% – 110%
Average Recovery	103%

ELISA Kit Detail Information

Related Target	AHSG
Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	100 μL
Sensitivity	2.22 pg/mL
Array Range	46.88 pg/mL – 3000 pg/mL
Assay Time	3.5 h
Recovery	88% – 110%
Average Recovery	103%
Intra Precision	4.9% – 5.1%
Inter Precision	2.3% – 5.4%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Fetuin-A monoclonal antibody Human Fetuin-A freeze-dried standard Fetuin-A detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Fetuin-A antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Fetuin-A present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Fetuin-A in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

AHSG

AHSG Target Infomation Overview

Target Symbol: AHSG, alpha 2-HS glycoprotein
Gene Groups: Cystatins, type 4
Alias: FETUA; A2HS; HSGA
Alias Names: fetuin A

AHSG, alpha 2-HS glycoprotein Target Infomation by Species

Human
Mouse
Rat

Human AHSG Target Information

Target Symbol: AHSG, alpha 2-HS glycoprotein
Alias:
- A2HS
- AHS
- alpha-2-HS-glycoprotein
- alpha-2-Z-globulin
- APMR1
- ba-alpha-2-glycoprotein
- FETUA
- fetuin A
- fetuin-A
- HSGA
NCBI_Gene: 197
UniProtKB: P02765

Human AHSG Predicted Functions

Predicted to enable endopeptidase inhibitor activity. Involved in acute-phase response; positive regulation of phagocytosis; and regulation of inflammatory response. Located in Golgi apparatus. Implicated in alopecia-mental retardation syndrome 1; coronary artery disease; and type 2 diabetes mellitus. Biomarker of gestational diabetes; myocardial infarction; obesity; and type 2 diabetes mellitus.

Mouse Ahsg Target Information

Target Symbol: Ahsg, alpha-2-HS-glycoprotein
Alias:
- fetuin-A
NCBI_Gene: 11625

Mouse Ahsg Predicted Functions

Predicted to enable endopeptidase inhibitor activity and receptor signaling protein tyrosine kinase inhibitor activity. Involved in negative regulation of bone mineralization. Acts upstream of or within ossification. Predicted to be located in Golgi apparatus; collagen-containing extracellular matrix; and extracellular space. Predicted to be part of protein-containing complex. Predicted to be active in extracellular matrix and extracellular region. Is expressed in several structures, including gut; heart; limb segment; reproductive system; and skeletal musculature. Human ortholog(s) of this gene implicated in alopecia-mental retardation syndrome 1; coronary artery disease; and type 2 diabetes mellitus. Orthologous to human AHSG (alpha 2-HS glycoprotein).

Rat Ahsg Target Information

Target Symbol: Ahsg, alpha-2-HS-glycoprotein
Alias:
- 59 kDa bone sialic acid-containing protein
- Aa2-066
- alpha 2 HS-glycoprotein alpha 2 (fetuin)
- BSP
- fetuin-A
- glycoprotein PP63
- phosphorylated N-glycoprotein pp63
- phosphorylated N-glycoprotein, pp63
- pp63
NCBI_Gene: 25373
UniProtKB: P24090

Rat Ahsg Predicted Functions

Enables receptor signaling protein tyrosine kinase inhibitor activity. Involved in several processes, including animal organ development; negative regulation of bone mineralization; and positive regulation of bone resorption. Located in collagen-containing extracellular matrix and extracellular space. Part of protein-containing complex. Human ortholog(s) of this gene implicated in alopecia-mental retardation syndrome 1; coronary artery disease; and type 2 diabetes mellitus. Orthologous to human AHSG (alpha 2-HS glycoprotein).

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