EK1151
Human FGF-21 ELISA Kit
$245.00 – $350.00
Related Target | |
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Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 50 μL |
Sensitivity | 0.19 pg/mL |
Array Range | 31.25 pg/mL – 2000 pg/mL |
Assay Time | 3.5 h |
Recovery | 81% – 121% |
Average Recovery | 104% |
ELISA Kit Detail Information
Related Target | |
---|---|
Species | human |
Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
Sample Volume | 50 μL |
Sensitivity | 0.19 pg/mL |
Array Range | 31.25 pg/mL – 2000 pg/mL |
Assay Time | 3.5 h |
Recovery | 81% – 121% |
Average Recovery | 104% |
Intra Precision | 2.7% – 4.8% |
Inter Precision | 3.1% – 6.1% |
Plate | Detachable 96-well plate |
Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
Delivery | 4℃ blue ice transportation |
Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-FGF-21 monoclonal antibody Human FGF-21 freeze-dried standard FGF-21 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human FGF-21 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, FGF-21 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of FGF-21 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
FGF21
No description available.
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