Human FGF-basic High Sensitivity ELISA Kit

$380.00$480.00

Download:
TARGETSFGF2
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.04 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery86% – 97%
Average Recovery0.9
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample VolumeSerum, plasma: 20 μL;cell culture supernatant: 100 μL
Sensitivity0.04 pg/mL
Range7.81 pg/mL – 500 pg/mL
Assay Time3.5 h
Recovery86% – 97%
Average Recovery0.9
Intra Precision3.2% – 5.2%
Inter-Precision3.5% – 6.4%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-FGF-basic monoclonal antibody
Human FGF-basic freeze-dried standard
FGF-basic detect Antibody
Standard Diluent
HRP-labeled streptavidin
Signal enhancer concentrate
Signal enhancer diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human FGF-basic antibodies are precoated on a high-affinity ELISA plate.Standards and test samples are added to the wells of the ELISA plate. After incubation, the FGF-basic present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, biotinylated detection antibodies are added and incubated. After washing to remove unbound biotinylated antibodies, streptavidin-HRP labeled with horseradish peroxidase is added. After washing again, a signal enhancer is added and incubated. After washing to remove unbound substances, Streptavidin-HRP is added once more. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of FGF-basic in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

FGF2

FGF2 Target Infomation Overview

  • Target Symbol: FGF2, fibroblast growth factor 2
  • Gene Groups: Receptor ligands; Fibroblast growth factor family
  • Previous Names: FGFB
  • Alias Names: fibroblast growth factor 2 (basic)

FGF2, fibroblast growth factor 2 Target Infomation by Species

HumanMouseRat

Human FGF2 Target Information

  • Target Symbol: FGF2, fibroblast growth factor 2
  • Alias:
    • basic fibroblast growth factor bFGF
    • BFGF
    • FGF-2
    • FGFB
    • fibroblast growth factor 2 (basic)
    • HBGF-2
    • heparin-binding growth factor 2
    • prostatropin
  • NCBI_Gene: 2247
  • UniProtKB: P09038

Human FGF2 Predicted Functions

Enables several functions, including identical protein binding activity; nuclear receptor coactivator activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and regulation of cell migration. Acts upstream of or within positive regulation of cell fate specification and positive regulation of cell population proliferation. Located in extracellular space. Implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Biomarker of several diseases, including artery disease (multiple); breast cancer (multiple); carcinoma (multiple); eye disease (multiple); and female reproductive organ cancer (multiple).

Mouse Fgf2 Target Information

Mouse Fgf2 Predicted Functions

Enables growth factor activity. Involved in stem cell development. Acts upstream of or within several processes, including animal organ development; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).

Rat Fgf2 Target Information

Rat Fgf2 Predicted Functions

Enables fibroblast growth factor receptor binding activity. Involved in several processes, including angiogenesis involved in coronary vascular morphogenesis; paracrine signaling; and positive regulation of cell population proliferation. Acts upstream of or within fibroblast growth factor receptor signaling pathway. Located in cytoplasm; extracellular space; and nucleus. Used to study pulmonary hypertension. Biomarker of bladder disease; decubitus ulcer; diabetic retinopathy; hypertension; and retinal vein occlusion. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).

FGF2 Target News

    11.061
    3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis
    Biofabrication
    Osteoarthrosis & Rheumatoid Arthritis

    Related Products

    more +

    $380.00$480.00

    $380.00$480.00

    $380.00$480.00

    Same Target

    EK1F03

    $350.00$450.00