Powered by BiozMouse FGF basic/FGF2/bFGF ELISA Kit
$299.00 – $419.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples |
| Sample Volume | Serum, plasma: 10 μL, cell culture supernatant: 100 μL |
| Sensitivity | 27.11 pg/mL |
| Array Range | 46.87 pg/mL - 3000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% - 114% |
| Average Recovery | 101% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, DMEM cell culture supernatant and other biological samples |
| Sample Volume | Serum, plasma: 10 μL, cell culture supernatant: 100 μL |
| Sensitivity | 27.11 pg/mL |
| Array Range | 46.87 pg/mL - 3000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 85% - 114% |
| Average Recovery | 101% |
| Intra Precision | 8.9% - 9.2% |
| Inter Precision | 2.2% - 6.3% |
| Plate | Detachable 96-well Plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ Blue Ice Transport |
| Components | 1× Washing Buffer 1× Assay Buffer Stop Solution Substrate TMB Detect Antibody Standard Diluent Streptavidin-HRP Standard Microplate Wells |
| Assay Principle | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse FGF basic has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for FGF basic are pipetted into the wells and FGF basic present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Related Targets
FGF2
FGF2 Target Infomation Overview
- Target Symbol: FGF2, fibroblast growth factor 2
- Gene Groups: Receptor ligands; Fibroblast growth factor family
- Previous Names: FGFB
- Alias Names: fibroblast growth factor 2 (basic)
FGF2, fibroblast growth factor 2 Target Infomation by Species
- Human
- Mouse
- Rat
Human FGF2 Target Information
- Target Symbol: FGF2, fibroblast growth factor 2
- Alias:
- basic fibroblast growth factor bFGF
- BFGF
- FGF-2
- FGFB
- fibroblast growth factor 2 (basic)
- HBGF-2
- heparin-binding growth factor 2
- prostatropin
- NCBI_Gene: 2247
- UniProtKB: P09038
Human FGF2 Predicted Functions
Enables several functions, including identical protein binding activity; nuclear receptor coactivator activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and regulation of cell migration. Acts upstream of or within positive regulation of cell fate specification and positive regulation of cell population proliferation. Located in extracellular space. Implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Biomarker of several diseases, including artery disease (multiple); breast cancer (multiple); carcinoma (multiple); eye disease (multiple); and female reproductive organ cancer (multiple).
Mouse Fgf2 Target Information
- Target Symbol: Fgf2, fibroblast growth factor 2
- Alias:
- bFGF
- Fgf-2
- Fgfb
- fibroblast growth factor, basic
- NCBI_Gene: 14173
Mouse Fgf2 Predicted Functions
Enables growth factor activity. Involved in stem cell development. Acts upstream of or within several processes, including animal organ development; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).
Rat Fgf2 Target Information
- Target Symbol: Fgf2, fibroblast growth factor 2
- Alias:
- angiogenesis promoting growth factor
- basic fibroblast growth factor
- bFGF
- Fgf-2
- Fgf2a
- fibroblast growth factor 2a
- HBGF-2
- heparin binding growth factor
- heparin-binding growth factor 2
- NCBI_Gene: 54250
Rat Fgf2 Predicted Functions
Enables fibroblast growth factor receptor binding activity. Involved in several processes, including angiogenesis involved in coronary vascular morphogenesis; paracrine signaling; and positive regulation of cell population proliferation. Acts upstream of or within fibroblast growth factor receptor signaling pathway. Located in cytoplasm; extracellular space; and nucleus. Used to study pulmonary hypertension. Biomarker of bladder disease; decubitus ulcer; diabetic retinopathy; hypertension; and retinal vein occlusion. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).
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