EK1F03

Human FGF basic ELISA Kit

$245.00$350.00

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.39 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery92% – 119%
Average Recovery110%

ELISA Kit Detail Information

Related Target
Specieshuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume20 μL
Sensitivity0.39 pg/mL
Array Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery92% – 119%
Average Recovery110%
Intra Precision3.8% – 6.5%
Inter Precision5.3% – 10.2%
PlateDetachable 96-well plate
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-FGF basic monoclonal antibody
Human FGF basic freeze-dried standard
FGF basic detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(22×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human FGF basic antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, FGF basic present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of FGF basic in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

Related Targets

FGF2

FGF2 Target Infomation Overview

  • Target Symbol: FGF2, fibroblast growth factor 2
  • Gene Groups: Receptor ligands; Fibroblast growth factor family
  • Previous Names: FGFB
  • Alias Names: fibroblast growth factor 2 (basic)

FGF2, fibroblast growth factor 2 Target Infomation by Species

  • Human
  • Mouse
  • Rat

Human FGF2 Target Information

  • Target Symbol: FGF2, fibroblast growth factor 2
  • Alias:
    • basic fibroblast growth factor bFGF
    • BFGF
    • FGF-2
    • FGFB
    • fibroblast growth factor 2 (basic)
    • HBGF-2
    • heparin-binding growth factor 2
    • prostatropin
  • NCBI_Gene: 2247
  • UniProtKB: P09038

Human FGF2 Predicted Functions

Enables several functions, including identical protein binding activity; nuclear receptor coactivator activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and regulation of cell migration. Acts upstream of or within positive regulation of cell fate specification and positive regulation of cell population proliferation. Located in extracellular space. Implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Biomarker of several diseases, including artery disease (multiple); breast cancer (multiple); carcinoma (multiple); eye disease (multiple); and female reproductive organ cancer (multiple).

Mouse Fgf2 Target Information

Mouse Fgf2 Predicted Functions

Enables growth factor activity. Involved in stem cell development. Acts upstream of or within several processes, including animal organ development; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; extraembryonic component; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).

Rat Fgf2 Target Information

Rat Fgf2 Predicted Functions

Enables fibroblast growth factor receptor binding activity. Involved in several processes, including angiogenesis involved in coronary vascular morphogenesis; paracrine signaling; and positive regulation of cell population proliferation. Acts upstream of or within fibroblast growth factor receptor signaling pathway. Located in cytoplasm; extracellular space; and nucleus. Used to study pulmonary hypertension. Biomarker of bladder disease; decubitus ulcer; diabetic retinopathy; hypertension; and retinal vein occlusion. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).

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