Product Category
Newest Products
Human Granzyme A ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 0.31 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 80% – 124% |
| Average Recovery | 1.12 |
| Intra Precision | 2.6% – 3.5% |
| Inter-Precision | 3.0% – 5.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Granzyme A monoclonal antibody Human Granzyme A freeze-dried standard Granzyme A detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human Granzyme A antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, Granzyme A present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of Granzyme A in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
GZMA
GZMA Target Infomation Overview
- Target Symbol: GZMA, granzyme A
- Gene Groups: Granule associated serine proteases of immune defence
- Previous Names: HFSP; CTLA3
- Alias Names: CTL tryptase; Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease); granzyme 1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
GZMA, granzyme A Target Infomation by Species
HumanMouseRat
Human GZMA Target Information
- Target Symbol: GZMA, granzyme A
- Alias:
- CTL tryptase
- CTLA3
- cytotoxic T-lymphocyte proteinase 1
- cytotoxic T-lymphocyte-associated serine esterase-3
- fragmentin-1
- granzyme 1
- Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3, Hanukah factor serine protease)
- granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
- granzyme-1
- h factor
- hanukah factor serine protease
- Hanukah factor serine protease)
- hanukkah factor
- HF
- HFSP
- NCBI_Gene: 3001
- UniProtKB: P12544
Human GZMA Predicted Functions
Enables protein homodimerization activity and serine-type endopeptidase activity. Involved in several processes, including cytotoxic T cell pyroptotic process; granzyme-mediated programmed cell death signaling pathway; and negative regulation of endodeoxyribonuclease activity. Predicted to be located in immunological synapse and nucleus. Biomarker of chronic obstructive pulmonary disease.
Mouse Gzma Target Information
- Target Symbol: Gzma, granzyme A
- Alias:
- AW494114
- BLT esterase
- Ctla-3
- Ctla3
- cytotoxic T lymphocyte-associated protein 3
- expressed sequence AW494114
- H factor
- Hanukah factor
- Hf
- SE1
- serine esterase 1
- TSP-1
- TSP1
- NCBI_Gene: 14938
Mouse Gzma Predicted Functions
Enables serine-type peptidase activity. Acts upstream of or within response to bacterium. Located in nucleus. Is expressed in thymus primordium. Orthologous to human GZMA (granzyme A).
Rat Gzma Target Information
- Target Symbol: Gzma, granzyme A
- Alias:
- 无
- NCBI_Gene: 266708
Rat Gzma Predicted Functions
Predicted to enable protein homodimerization activity and serine-type endopeptidase activity. Predicted to be involved in several processes, including cytotoxic T cell pyroptotic process; granzyme-mediated programmed cell death signaling pathway; and negative regulation of endodeoxyribonuclease activity. Predicted to act upstream of or within response to bacterium. Predicted to be located in nucleus. Orthologous to human GZMA (granzyme A).
