Human IFN-β ELISA Kit

$350.00$450.00

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TARGETSIFNB1
SPECIESHuman
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.19 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery79% – 95%
Average Recovery0.88
Sample TypeSerum, plasma, cell culture supernatant, and other biological samples
Sample Volume50 μL
Sensitivity3.19 pg/mL
Range15.63 pg/mL – 1000 pg/mL
Assay Time3.5 h
Recovery79% – 95%
Average Recovery0.88
Intra Precision4.5% – 5.0%
Inter-Precision3.6% – 7.9%
PlatformELISA
PlateDetachable 96-well plate
Size96T/48T
StorageIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery4℃ blue ice transportation
Components96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-β monoclonal antibody
Human IFN-β freeze-dried standard
IFN-β detect Antibody
Standard Diluent
Assay Buffer(10×)
Substrate TMB
Stop Solution
Washing Buffer(20×)
Sealing Film
Assay PrincipleThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IFN-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IFN-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

 

Targets

IFNB1

IFNB1 Target Infomation Overview

  • Target Symbol: IFNB1, interferon beta 1
  • Gene Groups: Interferons
  • Alias: IFB; IFF
  • Previous Names: IFNB
  • Alias Names: interferon, beta 1, fibroblast

IFNB1, interferon beta 1 Target Infomation by Species

HumanMouseRat

Human IFNB1 Target Information

  • Target Symbol: IFNB1, interferon beta 1
  • Alias:
    • fibroblast interferon
    • IFB
    • IFF
    • IFN-beta
    • IFNB
    • interferon beta
    • interferon-beta
    • interferon, beta 1
    • interferon, beta 1, fibroblast
    • MGC96956
  • NCBI_Gene: 3456
  • UniProtKB: P01574

Human IFNB1 Predicted Functions

Enables chloramphenicol O-acetyltransferase activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; negative regulation of T-helper 2 cell cytokine production; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within response to exogenous dsRNA. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in COVID-19.

Mouse Ifnb1 Target Information

Mouse Ifnb1 Predicted Functions

Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).

Rat Ifnb1 Target Information

Rat Ifnb1 Predicted Functions

Predicted to enable chloramphenicol O-acetyltransferase activity; cytokine activity; and type I interferon receptor binding activity. Involved in macrophage activation involved in immune response. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).

IFNB1 Target News

    4.4
    The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1
    JOURNAL OF IMMUNOLOGY
    7.046
    IFN-beta and EIF2AK2 are potential biomarkers for interstitial lung disease in anti-MDA5 positive dermatomyositis
    RHEUMATOLOGY
    5
    Luteolin-7-O-glucoside promotes macrophage release of IFN-β by maintaining mitochondrial function and corrects the disorder of glucose metabolism during RSV infection
    EUROPEAN JOURNAL OF PHARMACOLOGY
    Lung Disease (Asthma & COPD)
    Multiple Sclerosis
    Osteoarthrosis & Rheumatoid Arthritis
    Sepsis
    Systemic Lupus Erythematosus

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