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Human IFN-β ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 50 μL |
| Sensitivity | 3.19 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 79% – 95% |
| Average Recovery | 0.88 |
| Intra Precision | 4.5% – 5.0% |
| Inter-Precision | 3.6% – 7.9% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IFN-β monoclonal antibody Human IFN-β freeze-dried standard IFN-β detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IFN-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IFN-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-β in the samples. A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IFNB1
IFNB1 Target Infomation Overview
- Target Symbol: IFNB1, interferon beta 1
- Gene Groups: Interferons
- Alias: IFB; IFF
- Previous Names: IFNB
- Alias Names: interferon, beta 1, fibroblast
IFNB1, interferon beta 1 Target Infomation by Species
HumanMouseRat
Human IFNB1 Target Information
- Target Symbol: IFNB1, interferon beta 1
- Alias:
- fibroblast interferon
- IFB
- IFF
- IFN-beta
- IFNB
- interferon beta
- interferon-beta
- interferon, beta 1
- interferon, beta 1, fibroblast
- MGC96956
- NCBI_Gene: 3456
- UniProtKB: P01574
Human IFNB1 Predicted Functions
Enables chloramphenicol O-acetyltransferase activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; negative regulation of T-helper 2 cell cytokine production; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within response to exogenous dsRNA. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in COVID-19.
Mouse Ifnb1 Target Information
- Target Symbol: Ifnb1, interferon beta 1, fibroblast
- Alias:
- Ifb
- IFN-beta
- IFNB
- NCBI_Gene: 15977
Mouse Ifnb1 Predicted Functions
Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
Rat Ifnb1 Target Information
- Target Symbol: Ifnb1, interferon beta 1
- Alias:
- If1da1
- IFN-beta
- Ifnb
- interferon 1da1
- interferon beta
- Interferon beta 1 fibroblast
- interferon beta 1, fibroblast
- interferon, beta 1
- Interferon, beta 1, fibroblast
- NCBI_Gene: 24481
Rat Ifnb1 Predicted Functions
Predicted to enable chloramphenicol O-acetyltransferase activity; cytokine activity; and type I interferon receptor binding activity. Involved in macrophage activation involved in immune response. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
IFNB1 Target News
4.4
The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1
JOURNAL OF IMMUNOLOGY
7.046
IFN-beta and EIF2AK2 are potential biomarkers for interstitial lung disease in anti-MDA5 positive dermatomyositis
RHEUMATOLOGY
5
Luteolin-7-O-glucoside promotes macrophage release of IFN-β by maintaining mitochondrial function and corrects the disorder of glucose metabolism during RSV infection
EUROPEAN JOURNAL OF PHARMACOLOGY
