Mouse IFN-β ELISA Kit
$245.00 – $350.00
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 0.83 pg/mL |
| Array Range | 15.63 pg/mL – 1,000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 109% |
| Average Recovery | 103% |
ELISA Kit Detail Information
| Related Target | |
|---|---|
| Species | mouse |
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
| Sample Volume | 20 μL |
| Sensitivity | 0.83 pg/mL |
| Array Range | 15.63 pg/mL – 1,000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 91% – 109% |
| Average Recovery | 103% |
| Intra Precision | 3.1% – 4.7% |
| Inter Precision | 3.5% – 4.3% |
| Plate | Detachable 96-well plate |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Mouse IFN-β monoclonal antibody Mouse IFN-β freeze-dried standard Mouse IFN-β detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-mouse IFN-β antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IFN-β present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IFN-β in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Related Targets
IFNB1
IFNB1 Target Infomation Overview
- Target Symbol: IFNB1, interferon beta 1
- Gene Groups: Interferons
- Alias: IFB; IFF
- Previous Names: IFNB
- Alias Names: interferon, beta 1, fibroblast
IFNB1, interferon beta 1 Target Infomation by Species
- Human
- Mouse
- Rat
Human IFNB1 Target Information
- Target Symbol: IFNB1, interferon beta 1
- Alias:
- fibroblast interferon
- IFB
- IFF
- IFN-beta
- IFNB
- interferon beta
- interferon-beta
- interferon, beta 1
- interferon, beta 1, fibroblast
- MGC96956
- NCBI_Gene: 3456
- UniProtKB: P01574
Human IFNB1 Predicted Functions
Enables chloramphenicol O-acetyltransferase activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; negative regulation of T-helper 2 cell cytokine production; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within response to exogenous dsRNA. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in COVID-19.
Mouse Ifnb1 Target Information
- Target Symbol: Ifnb1, interferon beta 1, fibroblast
- Alias:
- Ifb
- IFN-beta
- IFNB
- NCBI_Gene: 15977
Mouse Ifnb1 Predicted Functions
Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
Rat Ifnb1 Target Information
- Target Symbol: Ifnb1, interferon beta 1
- Alias:
- If1da1
- IFN-beta
- Ifnb
- interferon 1da1
- interferon beta
- Interferon beta 1 fibroblast
- interferon beta 1, fibroblast
- interferon, beta 1
- Interferon, beta 1, fibroblast
- NCBI_Gene: 24481
Rat Ifnb1 Predicted Functions
Predicted to enable chloramphenicol O-acetyltransferase activity; cytokine activity; and type I interferon receptor binding activity. Involved in macrophage activation involved in immune response. Predicted to be active in extracellular space. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).
Products with the Same Target - IFNB1
Citations for Mouse IFN-β ELISA Kit
There are no citations for this product yet.
If you buy this product and mention it in your thesis, we welcome you to provide us with the thesis information. As a thank you, we will give you some gifts, such as discount coupons.
Citations data is updating...
Competitive ELISA: Principles, Methods, and Key Differences
Competitive ELISA is a widely used immunoassay technique for quantifying target analytes in samples by measuring their interference with a known signal. This method is based on the principle of limited antibodies, where the target analyte competes with a labeled antigen for antibody binding. Competitive ELISA is commonly used for detecting small molecules with fewer epitopes and is available in direct and indirect formats. This article explains the fundamental principles, experimental workflow, and key differences between direct and indirect competitive ELISA, helping researchers choose the optimal method for their applications.
ELISA (Enzyme-Linked Immunosorbent Assay): Principle, Types, and Step-by-Step Protocol
Learn about ELISA (Enzyme-Linked Immunosorbent Assay), a highly sensitive immunoassay technique used in medical diagnostics and biopharmaceuticals. This guide covers its principles, common types (sandwich, direct, indirect, competitive), detailed protocol, and key considerations for accurate results.
ELISA Blood Samples: Serum vs Plasma - Key Differences, Preparation & Hemolysis Effects
Learn whether to use serum or plasma for ELISA testing, including preparation protocols, key differences (fibrinogen, clotting factors), and how hemolysis impacts results. Essential guide for accurate immunoassays.
Related products
-
-
- EK1120
- ELISA Kit
Human Activin A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1292
- ELISA Kit
Human ANGPTL4 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1203
- ELISA Kit
Human Apolipoprotein B/Apo B ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1317
- ELISA Kit
Human ACE ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1109
- ELISA Kit
Human sTNF RI/TNFRSF1A ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
-
-
- EK1122
- ELISA Kit
Human Angiopoietin-1/ANG-1 ELISA Kit
- $245.00 – $350.00
- Select options This product has multiple variants. The options may be chosen on the product page
-
Reviews
There are no reviews yet.