Human IgA ELISA Kit

$245.00 – $350.00

Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity	0.35 ng/mL
Array Range	3.13 ng/mL – 200 ng/mL
Assay Time	3.5 h
Recovery	90% – 108%
Average Recovery	97%

ELISA Kit Detail Information

Species	human
Sample Type	Serum, plasma, cell culture supernatant, and other biological samples
Sample Volume	Serum, plasma: 10 μL, cell culture supernatant: 100 μL
Sensitivity	0.35 ng/mL
Array Range	3.13 ng/mL – 200 ng/mL
Assay Time	3.5 h
Recovery	90% – 108%
Average Recovery	97%
Intra Precision	3.4% – 4.8%
Inter Precision	0.7% – 6.8%
Plate	Detachable 96-well plate
Storage	If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.
Delivery	4℃ blue ice transportation
Components	96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IgA monoclonal antibody Human IgA freeze-dried standard IgA detect Antibody Standard Diluent HRP-labeled streptavidin Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film
Assay Principle	This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IgA antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and the detection antibody labeled with horseradish peroxidase are added to the wells of the ELISA plate. After incubation, IgA present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgA in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).

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