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Human IGFBP-4 ELISA Kit
$350.00 – $450.00
| Sample Type | Serum, plasma, cell culture supernatant, and other biological samples |
|---|---|
| Sample Volume | 20 μL |
| Sensitivity | 6.51 pg/mL |
| Range | 125 pg/mL – 8000 pg/mL |
| Assay Time | 3.5 h |
| Recovery | 81% – 115% |
| Average Recovery | 0.94 |
| Intra Precision | 2.9% – 4.0% |
| Inter-Precision | 2.8% – 4.3% |
| Platform | ELISA |
| Plate | Detachable 96-well plate |
| Size | 96T/48T |
| Storage | If the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃. |
| Delivery | 4℃ blue ice transportation |
| Components | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-TRAIL/TNFSF10 monoclonal antibody Human TRAIL/TNFSF10 freeze-dried standard TRAIL/TNFSF10 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Assay Principle | This kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IGFBP-4 antibodies are precoated on a high-affinity ELISA plate. Standard samples, test samples, and biotinylated detection antibodies are added to the wells of the ELISA plate. After incubation, IGFBP-4 present in the samples binds to the solid-phase antibodies and the detection antibodies. After washing to remove unbound substances, streptavidin-HRP labeled with horseradish peroxidase is added. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IGFBP-4 in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm). |
Targets
IGFBP4
IGFBP4 Target Infomation Overview
- Target Symbol: IGFBP4, insulin like growth factor binding protein 4
- Gene Groups: Insulin like growth factor binding proteins
- Alias: IBP4; BP-4; HT29-IGFBP; IGFBP-4
- Alias Names: IGF-binding protein 4; insulin-like growth factor-binding protein 4; insulin-like growth factor binding protein 4
IGFBP4, insulin like growth factor binding protein 4 Target Infomation by Species
HumanMouseRat
Human IGFBP4 Target Information
- Target Symbol: IGFBP4, insulin like growth factor binding protein 4
- Alias:
- BP-4
- HT29-IGFBP
- IBP-4
- IBP4
- IGF-binding protein 4
- IGFBP-4
- insulin-like growth factor binding protein 4
- insulin-like growth factor-binding protein 4
- NCBI_Gene: 3487
- UniProtKB: P22692
Human IGFBP4 Predicted Functions
Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Predicted to be involved in regulation of insulin-like growth factor receptor signaling pathway. Predicted to act upstream of or within several processes, including positive regulation of signal transduction; regulation of glucose metabolic process; and type B pancreatic cell proliferation. Predicted to be located in endoplasmic reticulum lumen and extracellular region. Predicted to be active in extracellular space.
Mouse Igfbp4 Target Information
- Target Symbol: Igfbp4, insulin-like growth factor binding protein 4
- Alias:
- AI875747
- Band 3A
- Deb2
- differentially expressed in B16F10 2
- expressed sequence AI875747
- IGFBP-4
- NCBI_Gene: 16010
Mouse Igfbp4 Predicted Functions
Enables insulin-like growth factor binding activity. Acts upstream of or within several processes, including positive regulation of signal transduction; regulation of glucose metabolic process; and type B pancreatic cell proliferation. Located in extracellular space. Is expressed in several structures, including alimentary system; early embryo; female reproductive system; limb; and lung. Orthologous to human IGFBP4 (insulin like growth factor binding protein 4).
Rat Igfbp4 Target Information
- Target Symbol: Igfbp4, insulin-like growth factor binding protein 4
- Alias:
- IBP-4
- IBP4
- IGF-binding protein 4
- IGF-BP4
- IGFBP-4
- insulin-like growth factor-binding protein 4
- NCBI_Gene: 360622
- UniProtKB: P21744
Rat Igfbp4 Predicted Functions
Predicted to enable insulin-like growth factor I binding activity and insulin-like growth factor II binding activity. Involved in inflammatory response. Predicted to be active in extracellular space. Orthologous to human IGFBP4 (insulin like growth factor binding protein 4).
